A Comparison of the Proteomic Expression in Pooled Saliva Specimens from Individuals Diagnosed with Ductal Carcinoma of the Breast with and without Lymph Node Involvement
Joint Authors
Streckfus, Charles Frederick
Storthz, Karen A.
Dubinsky, William P.
Bigler, Lenora
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-12-20
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Purpose.
The objective was to compare the salivary protein profiles of saliva specimens from individuals diagnosed with invasive ductal carcinoma of the breast (IDC) with and without lymph node involvement.
Methods.
Three pooled saliva specimens from women were analyzed.
One pooled specimen was from healthy women; another was from women diagnosed with Stage IIa IDC and a specimen from women diagnosed with Stage IIb.
The pooled samples were trypsinized and the peptide digests labeled with the appropriate iTRAQ reagent.
Labeled peptides from each of the digests were combined and analyzed by reverse phase capillary chromatography on an LC-MS/MS mass spectrometer.
Results.
The results yielded approximately 174 differentially expressed proteins in the saliva specimens.
There were 55 proteins that were common to both cancer stages in comparison to each other and healthy controls while there were 20 proteins unique to Stage IIa and 28 proteins that were unique to Stage IIb.
American Psychological Association (APA)
Streckfus, Charles Frederick& Storthz, Karen A.& Bigler, Lenora& Dubinsky, William P.. 2009. A Comparison of the Proteomic Expression in Pooled Saliva Specimens from Individuals Diagnosed with Ductal Carcinoma of the Breast with and without Lymph Node Involvement. Journal of Oncology،Vol. 2009, no. 2009, pp.1-11.
https://search.emarefa.net/detail/BIM-494703
Modern Language Association (MLA)
Streckfus, Charles Frederick…[et al.]. A Comparison of the Proteomic Expression in Pooled Saliva Specimens from Individuals Diagnosed with Ductal Carcinoma of the Breast with and without Lymph Node Involvement. Journal of Oncology No. 2009 (2009), pp.1-11.
https://search.emarefa.net/detail/BIM-494703
American Medical Association (AMA)
Streckfus, Charles Frederick& Storthz, Karen A.& Bigler, Lenora& Dubinsky, William P.. A Comparison of the Proteomic Expression in Pooled Saliva Specimens from Individuals Diagnosed with Ductal Carcinoma of the Breast with and without Lymph Node Involvement. Journal of Oncology. 2009. Vol. 2009, no. 2009, pp.1-11.
https://search.emarefa.net/detail/BIM-494703
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-494703