Rottlerin Inhibits ROS Formation and Prevents NFκB Activation in MCF-7 and HT-29 Cells
Joint Authors
Maioli, Emanuela
Greci, Lucedio
Pecorelli, Alessandra
Fortino, Vittoria
Souček, Karel
Giuseppe, Valacchi
Hyzdalova, Martina
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-02-03
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Rottlerin, a polyphenol isolated from Mallotus Philippinensis, has been recently used as a selective inhibitor of PKC δ, although it can inhibit many kinases and has several biological effects.
Among them, we recently found that Rottlerin inhibits the Nuclear Factor κB (NFκB), activated by either phorbol esters or H2O2.
Because of the redox sensitivity of NFκB and on the basis of Rottlerin antioxidant property, we hypothesized that Rottlerin could prevent NFκB activation acting as a free radicals scavenger, as other natural polyphenols.
The current study confirms the antioxidant property of Rottlerin against the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) in vitro and against oxidative stress induced by H2O2 and by menadione in culture cells.
We also demonstrate that Rottlerin prevents TNFα-dependent NFκB activation in MCF-7 cells and in HT-29 cells transfected with the NFκB-driven plasmid pBIIX-LUC, suggesting that Rottlerin can inhibit NFκB via several pathways and in several cell types.
American Psychological Association (APA)
Maioli, Emanuela& Greci, Lucedio& Souček, Karel& Hyzdalova, Martina& Pecorelli, Alessandra& Fortino, Vittoria…[et al.]. 2010. Rottlerin Inhibits ROS Formation and Prevents NFκB Activation in MCF-7 and HT-29 Cells. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-495191
Modern Language Association (MLA)
Maioli, Emanuela…[et al.]. Rottlerin Inhibits ROS Formation and Prevents NFκB Activation in MCF-7 and HT-29 Cells. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-7.
https://search.emarefa.net/detail/BIM-495191
American Medical Association (AMA)
Maioli, Emanuela& Greci, Lucedio& Souček, Karel& Hyzdalova, Martina& Pecorelli, Alessandra& Fortino, Vittoria…[et al.]. Rottlerin Inhibits ROS Formation and Prevents NFκB Activation in MCF-7 and HT-29 Cells. Journal of Biomedicine and Biotechnology. 2010. Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-495191
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495191