Choline Modulation of the Aβ P1-40 Channel Reconstituted into a Model Lipid Membrane

Joint Authors

Micelli, Silvia
Meleleo, Daniela
Notarachille, Gabriella

Source

International Journal of Alzheimer's Disease

Issue

Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-12, 12 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2011-01-03

Country of Publication

Egypt

No. of Pages

12

Main Subjects

Diseases
Medicine

Abstract EN

Nicotinic acetylcholine receptors (AChRs), implicated in memory and learning, in subjects affected by Alzheimer's disease result altered.

Stimulation of α7-nAChRs inhibits amyloid plaques and increases ACh release.

β-amyloid peptide (AβP) forms ion channels in the cell and model phospholipid membranes that are retained responsible in Alzheimer disease.

We tested if choline, precursor of ACh, could affect the AβP1-40 channels in oxidized cholesterol (OxCh) and in palmitoyl-oleoyl-phosphatidylcholine (POPC):Ch lipid bilayers.

Choline concentrations of 5 × 10−11 M–1.5 × 10−8 M added to the cis- or trans-side of membrane quickly increased AβP1-40 ion channel frequency (events/min) and ion conductance in OxCh membranes, but not in POPC:Ch membranes.

Circular Dichroism (CD) spectroscopy shows that after 24 and 48 hours of incubation with AβP1-40, choline stabilizes the random coil conformation of the peptide, making it less prone to fibrillate.

These actions seem to be specific in that ACh is ineffective either in solution or on AβP1-40 channel incorporated into PLMs.

American Psychological Association (APA)

Meleleo, Daniela& Notarachille, Gabriella& Micelli, Silvia. 2011. Choline Modulation of the Aβ P1-40 Channel Reconstituted into a Model Lipid Membrane. International Journal of Alzheimer's Disease،Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-495976

Modern Language Association (MLA)

Meleleo, Daniela…[et al.]. Choline Modulation of the Aβ P1-40 Channel Reconstituted into a Model Lipid Membrane. International Journal of Alzheimer's Disease No. 2010 (2010), pp.1-12.
https://search.emarefa.net/detail/BIM-495976

American Medical Association (AMA)

Meleleo, Daniela& Notarachille, Gabriella& Micelli, Silvia. Choline Modulation of the Aβ P1-40 Channel Reconstituted into a Model Lipid Membrane. International Journal of Alzheimer's Disease. 2011. Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-495976

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-495976