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Choline Modulation of the Aβ P1-40 Channel Reconstituted into a Model Lipid Membrane
Joint Authors
Micelli, Silvia
Meleleo, Daniela
Notarachille, Gabriella
Source
International Journal of Alzheimer's Disease
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-01-03
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Nicotinic acetylcholine receptors (AChRs), implicated in memory and learning, in subjects affected by Alzheimer's disease result altered.
Stimulation of α7-nAChRs inhibits amyloid plaques and increases ACh release.
β-amyloid peptide (AβP) forms ion channels in the cell and model phospholipid membranes that are retained responsible in Alzheimer disease.
We tested if choline, precursor of ACh, could affect the AβP1-40 channels in oxidized cholesterol (OxCh) and in palmitoyl-oleoyl-phosphatidylcholine (POPC):Ch lipid bilayers.
Choline concentrations of 5 × 10−11 M–1.5 × 10−8 M added to the cis- or trans-side of membrane quickly increased AβP1-40 ion channel frequency (events/min) and ion conductance in OxCh membranes, but not in POPC:Ch membranes.
Circular Dichroism (CD) spectroscopy shows that after 24 and 48 hours of incubation with AβP1-40, choline stabilizes the random coil conformation of the peptide, making it less prone to fibrillate.
These actions seem to be specific in that ACh is ineffective either in solution or on AβP1-40 channel incorporated into PLMs.
American Psychological Association (APA)
Meleleo, Daniela& Notarachille, Gabriella& Micelli, Silvia. 2011. Choline Modulation of the Aβ P1-40 Channel Reconstituted into a Model Lipid Membrane. International Journal of Alzheimer's Disease،Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-495976
Modern Language Association (MLA)
Meleleo, Daniela…[et al.]. Choline Modulation of the Aβ P1-40 Channel Reconstituted into a Model Lipid Membrane. International Journal of Alzheimer's Disease No. 2010 (2010), pp.1-12.
https://search.emarefa.net/detail/BIM-495976
American Medical Association (AMA)
Meleleo, Daniela& Notarachille, Gabriella& Micelli, Silvia. Choline Modulation of the Aβ P1-40 Channel Reconstituted into a Model Lipid Membrane. International Journal of Alzheimer's Disease. 2011. Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-495976
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495976