Terminally Differentiated Epithelial Cells of the Thymic Medulla and Skin Express Nicotinic Acetylcholine Receptor Subunit α3
Joint Authors
Soultanova, Aichurek
Rafiq, Amir
Kummer, Wolfgang
Panneck, Alexandra R.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-02
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
In the thymus, T cell maturation is influenced by cholinergic signaling, and the predominantly expressed receptor is the α3-subunit of nicotinic acetylcholine receptors, encoded by the chrna3 gene.
We here determined its cellular distribution utilizing an appropriate eGFP-expressing reporter mouse strain.
Neither T cells (CD4, CD8) nor mesenchymal cells (desmin-positive) expressed eGFP.
In the thymic medulla, eGFP-positive cells either were scattered or, more frequently, formed small clusters resembling Hassall’s corpuscles.
Immunolabeling revealed that these cells were indeed terminally differentiated epithelial cells expressing keratin 10 (K10) but neither typical cortical (K8, K18) nor medullary keratins (K5, K14).
These labeling patterns reflected those in the epidermis of the skin, where overlap of K10 and eGFP expression was seen in the stratum granulosum, whereas underlying basal cells displayed K5-immunoreactivity.
A substantial portion of thymic eGFP-positive cells was also immunoreactive to chromogranin A, a peptide previously reported in epidermal keratinocytes in the stratum granulosum.
Its fragment catestatin has multiple biological activities, including suppression of proinflammatory cytokine release from macrophages and inhibition of α3β4 nAChR.
The present findings suggest that its thymic production and/or release are under cholinergic control involving nAChR containing the α3-subunit.
American Psychological Association (APA)
Soultanova, Aichurek& Panneck, Alexandra R.& Rafiq, Amir& Kummer, Wolfgang. 2014. Terminally Differentiated Epithelial Cells of the Thymic Medulla and Skin Express Nicotinic Acetylcholine Receptor Subunit α3. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-496354
Modern Language Association (MLA)
Soultanova, Aichurek…[et al.]. Terminally Differentiated Epithelial Cells of the Thymic Medulla and Skin Express Nicotinic Acetylcholine Receptor Subunit α3. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-496354
American Medical Association (AMA)
Soultanova, Aichurek& Panneck, Alexandra R.& Rafiq, Amir& Kummer, Wolfgang. Terminally Differentiated Epithelial Cells of the Thymic Medulla and Skin Express Nicotinic Acetylcholine Receptor Subunit α3. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-496354
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-496354