Ethanolic Extract of Polish Propolis : Chemical Composition and TRAIL-R2 Death Receptor Targeting Apoptotic Activity against Prostate Cancer Cells
Joint Authors
Jaworska, Dagmara
Kucharska, Alicja Z.
Sokół-Łętowska, Anna
Szliszka, Ewelina
Czuba, Zenon P.
Krol, Wojciech
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-12
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Propolis possesses chemopreventive properties through direct anticancer and indirect immunomodulatory activities.
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) plays a significant role in immunosurveillance and defense against cancer cells.
TRAIL triggers apoptosis upon binding to TRAIL-R1 (DR4) and TRAIL-R2 (DR5) death receptors expressed on cancer cell surface.
The activation of TRAIL apoptotic signaling is considered an attractive option for cancer prevention.
However, as more tumor cells are reported to be resistant to TRAIL-mediated death, it is important to develop new strategies to overcome this resistance.
The aim of this study was to investigate the chemical composition and proapoptotic mechanism of ethanolic extract of Polish propolis (EEP-P) against cancer cells.
The identification and quantification of phenolic compounds in propolis extract were performed using HPLC-DAD and UPLC-Q-TOF-MS methods.
TRAIL-resistant LNCaP prostate cancer cells were treated with EEP-P and TRAIL.
Cytotoxicity was measured by MTT and LDH assays.
Apoptosis was detected using annexin V-FITC staining by flow cytometry and fluorescence microscopy.
Death receptors expression was analyzed using flow cytometry.
Pinobanksin, chrysin, methoxyflavanone, p-coumaric acid, ferulic acid and caffeic acid were the main phenolics found in EEP-P.
Propolis sensitized LNCaP cells through upregulation of TRAIL-R2.
These results suggest that EEP-P supports TRAIL-mediated immunochemoprevention in prostate cancer cells.
American Psychological Association (APA)
Szliszka, Ewelina& Sokół-Łętowska, Anna& Kucharska, Alicja Z.& Jaworska, Dagmara& Czuba, Zenon P.& Krol, Wojciech. 2013. Ethanolic Extract of Polish Propolis : Chemical Composition and TRAIL-R2 Death Receptor Targeting Apoptotic Activity against Prostate Cancer Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-496364
Modern Language Association (MLA)
Szliszka, Ewelina…[et al.]. Ethanolic Extract of Polish Propolis : Chemical Composition and TRAIL-R2 Death Receptor Targeting Apoptotic Activity against Prostate Cancer Cells. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-496364
American Medical Association (AMA)
Szliszka, Ewelina& Sokół-Łętowska, Anna& Kucharska, Alicja Z.& Jaworska, Dagmara& Czuba, Zenon P.& Krol, Wojciech. Ethanolic Extract of Polish Propolis : Chemical Composition and TRAIL-R2 Death Receptor Targeting Apoptotic Activity against Prostate Cancer Cells. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-496364
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-496364