Gene Expression Profiling of Placentas Affected by Pre-Eclampsia
Joint Authors
Borup, Rehannah
Sørensen, Steen
Hviid, Thomas V. F.
Hoegh, Anne Mette
Nielsen, Finn Cilius
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-03
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Several studies point to the placenta as the primary cause of pre-eclampsia.
Our objective was to identify placental genes that may contribute to the development of pre-eclampsia.
RNA was purified from tissue biopsies from eleven pre-eclamptic placentas and eighteen normal controls.
Messenger RNA expression from pooled samples was analysed by microarrays.
Verification of the expression of selected genes was performed using real-time PCR.
A surprisingly low number of genes (21 out of 15,000) were identified as differentially expressed.
Among these were genes not previously associated with pre-eclampsia as bradykinin B1 receptor and a 14-3-3 protein, but also genes that have already been connected with pre-eclampsia, for example, inhibin beta A subunit and leptin.
A low number of genes were repeatedly identified as differentially expressed, because they may represent the endpoint of a cascade of events effectuated throughout gestation.
They were associated with transcriptional regulation and vasoregulative pathways, along with a number of hypothetical proteins and gene sequences with unknown functions.
American Psychological Association (APA)
Hoegh, Anne Mette& Borup, Rehannah& Nielsen, Finn Cilius& Sørensen, Steen& Hviid, Thomas V. F.. 2010. Gene Expression Profiling of Placentas Affected by Pre-Eclampsia. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-498108
Modern Language Association (MLA)
Hoegh, Anne Mette…[et al.]. Gene Expression Profiling of Placentas Affected by Pre-Eclampsia. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-11.
https://search.emarefa.net/detail/BIM-498108
American Medical Association (AMA)
Hoegh, Anne Mette& Borup, Rehannah& Nielsen, Finn Cilius& Sørensen, Steen& Hviid, Thomas V. F.. Gene Expression Profiling of Placentas Affected by Pre-Eclampsia. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-498108
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-498108