Effects of Helicobacter pylori and Heat Shock Protein 70 on the Proliferation of Human Gastric Epithelial Cells
Joint Authors
Zou, Hai
Tao, Liping
Huang, Zhimin
Source
Gastroenterology Research and Practice
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-05
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Infection of Helicobacter pylori (H.
pylori) changed the proliferation of gastric epithelial cells and decreased the expression of heat shock protein 70 (HSP70).
However, the effects of H.
pylori on the proliferation of gastric epithelial cells and the roles of HSP70 during the progress need further investigation.
Objective.
To investigate the effects of Helicobacter pylori (H.
pylori) and heat shock protein 70 (HSP70) on the proliferation of human gastric epithelial cells.
Methods.
H.
pylori and a human gastric epithelial cell line (AGS) were cocultured.
The proliferation of AGS cells was quantitated by an MTT assay, and the expression of HSP70 in AGS cells was detected by Western blotting.
HSP70 expression in AGS cells was silenced by small interfering RNA (siRNA) to investigate the role of HSP70.
The siRNA-treated AGS cells were cocultured with H.
pylori and cell proliferation was measured by an MTT assay.
Results.
The proliferation of AGS cells was accelerated by coculturing with H.
pylori for 4 and 8 h, but was suppressed at 24 and 48 h.
HSP70 expression was decreased in AGS cells infected by H.
pylori for 48 h.
The proliferation in HSP70-silenced AGS cells was inhibited after coculturing with H.
pylori for 24 and 48 h compared with the control group.
Conclusions.
Coculture of H.
pylori altered the proliferation of gastric epithelial cells and decreased HSP70 expression.
HSP70 knockdown supplemented the inhibitory effect of H.
pylori on proliferation of epithelial cells.
These results indicate that the effects of H.
pylori on the proliferation of gastric epithelial cells at least partially depend on the decreased expression of HSP70 induced by the bacterium.
American Psychological Association (APA)
Tao, Liping& Zou, Hai& Huang, Zhimin. 2014. Effects of Helicobacter pylori and Heat Shock Protein 70 on the Proliferation of Human Gastric Epithelial Cells. Gastroenterology Research and Practice،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-498675
Modern Language Association (MLA)
Tao, Liping…[et al.]. Effects of Helicobacter pylori and Heat Shock Protein 70 on the Proliferation of Human Gastric Epithelial Cells. Gastroenterology Research and Practice No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-498675
American Medical Association (AMA)
Tao, Liping& Zou, Hai& Huang, Zhimin. Effects of Helicobacter pylori and Heat Shock Protein 70 on the Proliferation of Human Gastric Epithelial Cells. Gastroenterology Research and Practice. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-498675
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-498675