Inclusion Complex of Zerumbone with Hydroxypropyl-β-Cyclodextrin Induces Apoptosis in Liver Hepatocellular HepG2 Cells via Caspase 8BID Cleavage Switch and Modulating Bcl2Bax Ratio
Joint Authors
Ng, Kuan Beng
Rahman, Heshu Sulaiman
Anasamy, Theebaa
Abdel Wahab, Siddig Ibrahim
Syam, Suvitha
Arbab, Ismail Adam
Eid, Eltayeb E. M.
Mohan, Syam
Sukari, Mohd Aspollah
Ali, Hapipah Mohd
Muhammad Nadzri, Nabilah
Kamalidehghan, Behnam
Abdul, Ahmad Bustamam
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-08
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Zerumbone (ZER) isolated from Zingiber zerumbet was previously encapsulated with hydroxypropyl-β-cyclodextrin (HPβCD) to enhance ZER’s solubility in water, thus making it highly tolerable in the human body.
The anticancer effects of this new ZER-HPβCD inclusion complex via apoptosis cell death were assessed in this study for the first time in liver hepatocellular cells, HepG2.
Apoptosis was ascertained by morphological study, nuclear stain, and sub-G1 cell population accumulation with G2/M arrest.
Further investigations showed the release of cytochrome c and loss of mitochondrial membrane potential, proving mitochondrial dysfunction upon the ZER-HPβCD treatment as well as modulating proapoptotic and anti-apototic Bcl-2 family members.
A significant increase in caspase 3/7, caspase 9, and caspase 8 was detected with the depletion of BID cleaved by caspase 8.
Collectively, these results prove that a highly soluble inclusion complex of ZER-HPβCD could be a promising anticancer agent for the treatment of hepatocellular carcinoma in humans.
American Psychological Association (APA)
Muhammad Nadzri, Nabilah& Abdul, Ahmad Bustamam& Sukari, Mohd Aspollah& Abdel Wahab, Siddig Ibrahim& Eid, Eltayeb E. M.& Mohan, Syam…[et al.]. 2013. Inclusion Complex of Zerumbone with Hydroxypropyl-β-Cyclodextrin Induces Apoptosis in Liver Hepatocellular HepG2 Cells via Caspase 8BID Cleavage Switch and Modulating Bcl2Bax Ratio. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-16.
https://search.emarefa.net/detail/BIM-499933
Modern Language Association (MLA)
Muhammad Nadzri, Nabilah…[et al.]. Inclusion Complex of Zerumbone with Hydroxypropyl-β-Cyclodextrin Induces Apoptosis in Liver Hepatocellular HepG2 Cells via Caspase 8BID Cleavage Switch and Modulating Bcl2Bax Ratio. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-16.
https://search.emarefa.net/detail/BIM-499933
American Medical Association (AMA)
Muhammad Nadzri, Nabilah& Abdul, Ahmad Bustamam& Sukari, Mohd Aspollah& Abdel Wahab, Siddig Ibrahim& Eid, Eltayeb E. M.& Mohan, Syam…[et al.]. Inclusion Complex of Zerumbone with Hydroxypropyl-β-Cyclodextrin Induces Apoptosis in Liver Hepatocellular HepG2 Cells via Caspase 8BID Cleavage Switch and Modulating Bcl2Bax Ratio. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-16.
https://search.emarefa.net/detail/BIM-499933
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499933