Andrographolide, a Novel NF-κB Inhibitor, Induces Vascular Smooth Muscle Cell Apoptosis via a Ceramide-p47phox-ROS Signaling Cascade
Joint Authors
Hsu, Ming-Jen
Chen, Yu-Ying
Hsieh, Cheng-Ying
Lee, Lin-Wen
Sheu, Joen-Rong
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-29
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Atherosclerosis is linked with the development of many cardiovascular complications.
Abnormal proliferation of vascular smooth muscle cells (VSMCs) plays a crucial role in the development of atherosclerosis.
Accordingly, the apoptosis of VSMCs, which occurs in the progression of vascular proliferation, may provide a beneficial strategy for managing cardiovascular diseases.
Andrographolide, a novel nuclear factor-κB inhibitor, is the most active and critical constituent isolated from the leaves of Andrographis paniculata.
Recent studies have indicated that andrographolide is a potential therapeutic agent for treating cancer through the induction of apoptosis.
In this study, the apoptosis-inducing activity and mechanisms in andrographolide-treated rat VSMCs were characterized.
Andrographolide significantly induced reactive oxygen species (ROS) formation, p53 activation, Bax, and active caspase-3 expression, and these phenomena were suppressed by pretreating the cells with N-acetyl-L-cysteine, a ROS scavenger, or diphenylene iodonium, a nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (Nox) inhibitor.
Furthermore, p47phox, a Nox subunit protein, was phosphorylated in andrographolide-treated rat VSMCs.
However, pretreatment with 3-O-methyl-sphingomyelin, a neutral sphingomyelinase inhibitor, significantly inhibited andrographolide-induced p47phox phosphorylation as well as Bax and active caspase-3 expression.
Our results collectively demonstrate that andrographolide-reduced cell viability can be attributed to apoptosis in VSMCs, and this apoptosis-inducing activity was associated with the ceramide-p47phox-ROS signaling cascade.
American Psychological Association (APA)
Chen, Yu-Ying& Hsu, Ming-Jen& Sheu, Joen-Rong& Lee, Lin-Wen& Hsieh, Cheng-Ying. 2013. Andrographolide, a Novel NF-κB Inhibitor, Induces Vascular Smooth Muscle Cell Apoptosis via a Ceramide-p47phox-ROS Signaling Cascade. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-500882
Modern Language Association (MLA)
Chen, Yu-Ying…[et al.]. Andrographolide, a Novel NF-κB Inhibitor, Induces Vascular Smooth Muscle Cell Apoptosis via a Ceramide-p47phox-ROS Signaling Cascade. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-500882
American Medical Association (AMA)
Chen, Yu-Ying& Hsu, Ming-Jen& Sheu, Joen-Rong& Lee, Lin-Wen& Hsieh, Cheng-Ying. Andrographolide, a Novel NF-κB Inhibitor, Induces Vascular Smooth Muscle Cell Apoptosis via a Ceramide-p47phox-ROS Signaling Cascade. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-500882
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-500882