Biological Assessment of a Calcium Silicate Incorporated Hydroxyapatite-Gelatin Nanocomposite : A Comparison to Decellularized Bone Matrix
Joint Authors
Lee, Dong Joon
Ko, Ching-Chang
Zhang, He
Hu, Wei-Shou
Padilla, Ricardo
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-26
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Our laboratory utilized biomimicry to develop a synthetic bone scaffold based on hydroxyapatite-gelatin-calcium silicate (HGCS).
Here, we evaluated the potential of HGCS scaffold in bone formation in vivo using the rat calvarial critical-sized defect (CSD).
Twelve Sprague-Dawley rats were randomized to four groups: control (defect only), decellularized bone matrix (DECBM), and HGCS with and without multipotent adult progenitor cells (MAPCs).
DECBM was prepared by removing all the cells using SDS and NH4OH.
After 12 weeks, the CSD specimens were harvested to evaluate radiographical, histological, and histomorphometrical outcomes.
The in vitro osteogenic effects of the materials were studied by focal adhesion, MTS, and alizarin red.
Micro-CT analysis indicated that the DECBM and the HGCS scaffold groups developed greater radiopaque areas than the other groups.
Bone regeneration, assessed using histological analysis and fluorochrome labeling, was the highest in the HGCS scaffold seeded with MAPCs.
The DECBM group showed limited osteoinductivity, causing a gap between the implant and host tissue.
The group grafted with HGCS+MAPCs resulting in twice as much new bone formation seems to indicate a role for effective bone regeneration.
In conclusion, the novel HGCS scaffold could improve bone regeneration and is a promising carrier for stem cell-mediated bone regeneration.
American Psychological Association (APA)
Lee, Dong Joon& Padilla, Ricardo& Zhang, He& Hu, Wei-Shou& Ko, Ching-Chang. 2014. Biological Assessment of a Calcium Silicate Incorporated Hydroxyapatite-Gelatin Nanocomposite : A Comparison to Decellularized Bone Matrix. BioMed Research International،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-502130
Modern Language Association (MLA)
Lee, Dong Joon…[et al.]. Biological Assessment of a Calcium Silicate Incorporated Hydroxyapatite-Gelatin Nanocomposite : A Comparison to Decellularized Bone Matrix. BioMed Research International No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-502130
American Medical Association (AMA)
Lee, Dong Joon& Padilla, Ricardo& Zhang, He& Hu, Wei-Shou& Ko, Ching-Chang. Biological Assessment of a Calcium Silicate Incorporated Hydroxyapatite-Gelatin Nanocomposite : A Comparison to Decellularized Bone Matrix. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-502130
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-502130