Cloning, Sequencing, and In Silico Analysis of β-Propeller Phytase Bacillus licheniformis Strain PB-13
Joint Authors
Verma, A. K.
Kumar, Vinod
Agrawal, Sanjeev
Sangwan, Punesh
Singh, Gopal
Source
Biotechnology Research International
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-24
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Information Technology and Computer Science
Abstract EN
β-Propeller phytases (BPPhy) are widely distributed in nature and play a major role in phytate-phosphorus cycling.
In the present study, a BPPhy gene from Bacillus licheniformis strain was expressed in E.
coli with a phytase activity of 1.15 U/mL and specific activity of 0.92 U/mg proteins.
The expressed enzyme represented a full length ORF “PhyPB13” of 381 amino acid residues and differs by 3 residues from the closest similar existing BPPhy sequences.
The PhyPB13 sequence was characterized in silico using various bioinformatic tools to better understand structural, functional, and evolutionary aspects of BPPhy class by multiple sequence alignment and homology search, phylogenetic tree construction, variation in biochemical features, and distribution of motifs and superfamilies.
In all sequences, conserved sites were observed toward their N-terminus and C-terminus.
Cysteine was not present in the sequence.
Overall, three major clusters were observed in phylogenetic tree with variation in biophysical characteristics.
A total of 10 motifs were reported with motif “1” observed in all 44 protein sequences and might be used for diversity and expression analysis of BPPhy enzymes.
This study revealed important sequence features of BPPhy and pave a way for determining catalytic mechanism and selection of phytase with desirable characteristics.
American Psychological Association (APA)
Kumar, Vinod& Singh, Gopal& Sangwan, Punesh& Verma, A. K.& Agrawal, Sanjeev. 2014. Cloning, Sequencing, and In Silico Analysis of β-Propeller Phytase Bacillus licheniformis Strain PB-13. Biotechnology Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-502481
Modern Language Association (MLA)
Kumar, Vinod…[et al.]. Cloning, Sequencing, and In Silico Analysis of β-Propeller Phytase Bacillus licheniformis Strain PB-13. Biotechnology Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-502481
American Medical Association (AMA)
Kumar, Vinod& Singh, Gopal& Sangwan, Punesh& Verma, A. K.& Agrawal, Sanjeev. Cloning, Sequencing, and In Silico Analysis of β-Propeller Phytase Bacillus licheniformis Strain PB-13. Biotechnology Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-502481
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-502481