SKIP Downregulation Increases TGF-β1-Induced Matrix Metalloproteinase-9 Production in Transformed Keratinocytes
Joint Authors
Villar, Victor
Kocić, Jelena
Krstić, Aleksandra
Santibanez, Juan F.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-17
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Diseases
Abstract EN
Transforming growth factor-beta (TGF-β1) is a potent inductor of matrix metalloproteinase-9 (MMP-9) in transformed cells.
Recently, Ski-interacting protein (SKIP) has been described as a regulator of TGF-β1 signal transduction, but its role in the induction of cell malignance by TGF-β1 has not been fully elucidated so far.
In the present study, we analyzed the role of SKIP on TGF-β1-induced MMP-9 production.
Mouse transformed keratinocytes (PDV) were stably transfected with SKIP antisense construct.
We observed that SKIP depletion provoked an enhancement in the expression of MMP-9 in response to TGF-β1 treatment.
The downregulation of SKIP produced an enhancement in TGF-β1-activated ERK1,2 MAP kinase as well as increased transactivation of downstream Elk1 transcription factor.
The increased MMP-9 production in response to TGF-β1 was dependent of MAPK activation as PD98059, an MEK inhibitor, reduced MMP-9 expression in SKIP antisense transfected cells.
Thus, we propose SKIP as a regulatory protein in TGF-β1-induced MMP-9 expression acting by controlling ERK1,2 signaling in transformed cells.
American Psychological Association (APA)
Kocić, Jelena& Villar, Victor& Krstić, Aleksandra& Santibanez, Juan F.. 2012. SKIP Downregulation Increases TGF-β1-Induced Matrix Metalloproteinase-9 Production in Transformed Keratinocytes. Scientifica،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-504200
Modern Language Association (MLA)
Kocić, Jelena…[et al.]. SKIP Downregulation Increases TGF-β1-Induced Matrix Metalloproteinase-9 Production in Transformed Keratinocytes. Scientifica No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-504200
American Medical Association (AMA)
Kocić, Jelena& Villar, Victor& Krstić, Aleksandra& Santibanez, Juan F.. SKIP Downregulation Increases TGF-β1-Induced Matrix Metalloproteinase-9 Production in Transformed Keratinocytes. Scientifica. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-504200
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-504200