In-Depth Global Analysis of Transcript Abundance Levels in Porcine Alveolar Macrophages Following Infection with Porcine Reproductive and Respiratory Syndrome Virus
Joint Authors
Laegreid, William W.
Kehrli, Marcus E.
Lager, Kelly M.
Harhay, Gregory P.
Neill, John D.
Miller, Laura C.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-01-12
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Porcine reproductive and respiratory syndrome virus (PRRSV) is a major pathogen of swine worldwide and causes considerable economic loss.
Identifying specific cell signaling or activation pathways that associate with variation in PRRSV replication and macrophage function may lead to identification of novel gene targets for the control of PRRSV infection.
Serial Analysis of Gene Expression (SAGE) was used to create and survey the transcriptome of in vitro mock-infected and PRRSV strain VR-2332-infected porcine alveolar macrophages (PAM) at 0, 6, 12, 16, and 24 hours after infection.
The transcriptome data indicated changes in transcript abundance occurring in PRRSV-infected PAMs over time after infection with more than 590 unique tags with significantly altered transcript abundance levels identified (P<.01).
Strikingly, innate immune genes (whose transcript abundances are typically altered in response to other pathogens or insults including IL-8, CCL4, and IL-1β) showed no or very little change at any time point following infection.
American Psychological Association (APA)
Miller, Laura C.& Neill, John D.& Harhay, Gregory P.& Lager, Kelly M.& Laegreid, William W.& Kehrli, Marcus E.. 2011. In-Depth Global Analysis of Transcript Abundance Levels in Porcine Alveolar Macrophages Following Infection with Porcine Reproductive and Respiratory Syndrome Virus. Advances in Virology،Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-504464
Modern Language Association (MLA)
Miller, Laura C.…[et al.]. In-Depth Global Analysis of Transcript Abundance Levels in Porcine Alveolar Macrophages Following Infection with Porcine Reproductive and Respiratory Syndrome Virus. Advances in Virology No. 2010 (2010), pp.1-12.
https://search.emarefa.net/detail/BIM-504464
American Medical Association (AMA)
Miller, Laura C.& Neill, John D.& Harhay, Gregory P.& Lager, Kelly M.& Laegreid, William W.& Kehrli, Marcus E.. In-Depth Global Analysis of Transcript Abundance Levels in Porcine Alveolar Macrophages Following Infection with Porcine Reproductive and Respiratory Syndrome Virus. Advances in Virology. 2011. Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-504464
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-504464