Simultaneous Screening of 24 Target Genes of Foodborne Pathogens in 35 Foodborne Outbreaks Using Multiplex Real-Time SYBR Green PCR Analysis
Joint Authors
Sugama, Kumiko
Iida, Natsuko
Kawase, Jun
Yamaguchi, Keiji
Etoh, Yoshiki
Yashiro, Shunshuke
Fukushima, Hiroshi
Source
International Journal of Microbiology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-09-28
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
A set of 8 multiplex real-time SYBR Green PCR (SG-PCR) assays including 3 target primers and an internal amplification control (IAC) primer was simultaneously evaluated in 3 h or less with regard to detection of 24 target genes of 23 foodborne pathogens in 7 stool specimens of foodborne outbreak using a 96-well reaction plate.
This assay, combined with DNA extraction (QIAamp DNA Stool Mini kit), offered detection of greater than 103-104 foodborne pathogens per g in stool specimens.
The products formed were identified using melting point temperature (Tm) curve analysis.
This assay was evaluated for the detection of foodborne pathogens in 33 out of 35 cases of foodborne outbreak, using 4 different PCR instruments in 5 different laboratories.
No interference from the multiplex real-time SG-PCR assay, including IAC, was observed in stool specimens in any analysis.
We found multiplex real-time SG-PCR assay for simultaneous detection of 24 target genes of foodborne pathogens to be comprehensive, rapid, inexpensive, accurate, of high selectivity, and good for screening probability.
American Psychological Association (APA)
Fukushima, Hiroshi& Kawase, Jun& Etoh, Yoshiki& Sugama, Kumiko& Yashiro, Shunshuke& Iida, Natsuko…[et al.]. 2010. Simultaneous Screening of 24 Target Genes of Foodborne Pathogens in 35 Foodborne Outbreaks Using Multiplex Real-Time SYBR Green PCR Analysis. International Journal of Microbiology،Vol. 2010, no. 2010, pp.1-18.
https://search.emarefa.net/detail/BIM-504517
Modern Language Association (MLA)
Fukushima, Hiroshi…[et al.]. Simultaneous Screening of 24 Target Genes of Foodborne Pathogens in 35 Foodborne Outbreaks Using Multiplex Real-Time SYBR Green PCR Analysis. International Journal of Microbiology No. 2010 (2010), pp.1-18.
https://search.emarefa.net/detail/BIM-504517
American Medical Association (AMA)
Fukushima, Hiroshi& Kawase, Jun& Etoh, Yoshiki& Sugama, Kumiko& Yashiro, Shunshuke& Iida, Natsuko…[et al.]. Simultaneous Screening of 24 Target Genes of Foodborne Pathogens in 35 Foodborne Outbreaks Using Multiplex Real-Time SYBR Green PCR Analysis. International Journal of Microbiology. 2010. Vol. 2010, no. 2010, pp.1-18.
https://search.emarefa.net/detail/BIM-504517
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-504517