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Quantitative, Label-Free Detection of the Aggregation of α-Synuclein Using Microcantilever Arrays Operated in a Liquid Environment
Joint Authors
Jensen, Jason
Grange, Wilfried
Farina, Margherita
Zuccheri, Giampaolo
Hegner, Martin
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-19
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Early detection of protein aggregation is of great importance in the field of neurodegenerative diseases.
The successful detection of the aggregation of the protein α-synuclein in a quantitative, label-free manner by functionalising a microcantilever with α-synuclein monomers and operating it in dynamic mode in the presence of α-synuclein monomers in solution is reported.
A total mass of 6 ng of α-synuclein was detected over 9 hours on the surface of the cantilever.
The result is compared to conventional fluorescence measurements of α-synuclein aggregation under similar conditions.
It is found that the label-free cantilever detection method requires a concentration of protein 50 times smaller than that of the current method and indicated potential for significantly faster response times.
American Psychological Association (APA)
Jensen, Jason& Farina, Margherita& Zuccheri, Giampaolo& Grange, Wilfried& Hegner, Martin. 2011. Quantitative, Label-Free Detection of the Aggregation of α-Synuclein Using Microcantilever Arrays Operated in a Liquid Environment. Journal of Sensors،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-505258
Modern Language Association (MLA)
Jensen, Jason…[et al.]. Quantitative, Label-Free Detection of the Aggregation of α-Synuclein Using Microcantilever Arrays Operated in a Liquid Environment. Journal of Sensors No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-505258
American Medical Association (AMA)
Jensen, Jason& Farina, Margherita& Zuccheri, Giampaolo& Grange, Wilfried& Hegner, Martin. Quantitative, Label-Free Detection of the Aggregation of α-Synuclein Using Microcantilever Arrays Operated in a Liquid Environment. Journal of Sensors. 2011. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-505258
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-505258