Quantifying Changes in the Cellular Thiol-Disulfide Status during Differentiation of B Cells into Antibody-Secreting Plasma Cells

Joint Authors

Hansen, Rosa E.
Otsu, Mieko
Braakman, Ineke
Winther, Jakob R.

Source

International Journal of Cell Biology

Issue

Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2013-09-24

Country of Publication

Egypt

No. of Pages

9

Main Subjects

Biology

Abstract EN

Plasma cells produce and secrete massive amounts of disulfide-containing antibodies.

To accommodate this load on the secretory machinery, the differentiation of resting B cells into antibody-secreting plasma cells is accompanied by a preferential expansion of the secretory compartments of the cells and by an up-regulation of enzymes involved in redox regulation and protein folding.

We have quantified the absolute levels of protein thiols, protein disulfides, and glutathionylated proteins in whole cells.

The results show that while the global thiol-disulfide state is affected to some extent by the differentiation, steady-state levels of glutathionylated protein thiols are less than 0.3% of the total protein cysteines, even in fully differentiated cells, and the overall protein redox state is not affected until late in differentiation, when large-scale IgM production is ongoing.

A general expansion of the ER does not affect global protein redox status until an extensive production of cargo proteins has started.

American Psychological Association (APA)

Hansen, Rosa E.& Otsu, Mieko& Braakman, Ineke& Winther, Jakob R.. 2013. Quantifying Changes in the Cellular Thiol-Disulfide Status during Differentiation of B Cells into Antibody-Secreting Plasma Cells. International Journal of Cell Biology،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-506484

Modern Language Association (MLA)

Hansen, Rosa E.…[et al.]. Quantifying Changes in the Cellular Thiol-Disulfide Status during Differentiation of B Cells into Antibody-Secreting Plasma Cells. International Journal of Cell Biology No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-506484

American Medical Association (AMA)

Hansen, Rosa E.& Otsu, Mieko& Braakman, Ineke& Winther, Jakob R.. Quantifying Changes in the Cellular Thiol-Disulfide Status during Differentiation of B Cells into Antibody-Secreting Plasma Cells. International Journal of Cell Biology. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-506484

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-506484