Fluorogenic Membrane Overlays to Enumerate Total and Fecal Escherichia coli and Total Vibrionaceae in Shellfish and Seawater
Joint Authors
Watson, Michael A.
Richards, Gary P.
Source
International Journal of Microbiology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-04-12
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
Three assays were developed to enumerate total and fecal Escherichia coli and total Vibrionaceae in shellfish, seawater, and other foods and environmental samples.
Assays involve membrane overlays of overnight colonies on nonselective agar plates to detect β-glucuronidase and lysyl aminopeptidase activities for E.
coli and Vibrionaceae, respectively.
Cellulose membranes containing the substrates 4-methylumbeferyl-β-D-glucuronide (MUG) produced a bright blue fluorescence when overlaid onto E.
coli, while L-lysyl-7-amino-4-trifluoromethylcoumarin produced green fluorescent foci when overlaid onto Vibrionaceae family members.
A multiplex assay was also developed for simultaneously enumerating total E.
coli and total Vibrionaceae in oysters and seawater.
Overall, 65% of overlaid E.
coli (non-O157:H7) were MUG-positive, compared with 62% as determined by the most-probable-number-MUG assay.
The overlays are rapid, simple, and cost effective for quantification purposes.
This research provides practical alternatives for monitoring bacterial indicators and potential pathogens in complex samples, including molluscan shellfish.
American Psychological Association (APA)
Richards, Gary P.& Watson, Michael A.. 2010. Fluorogenic Membrane Overlays to Enumerate Total and Fecal Escherichia coli and Total Vibrionaceae in Shellfish and Seawater. International Journal of Microbiology،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-507399
Modern Language Association (MLA)
Richards, Gary P.& Watson, Michael A.. Fluorogenic Membrane Overlays to Enumerate Total and Fecal Escherichia coli and Total Vibrionaceae in Shellfish and Seawater. International Journal of Microbiology No. 2010 (2010), pp.1-9.
https://search.emarefa.net/detail/BIM-507399
American Medical Association (AMA)
Richards, Gary P.& Watson, Michael A.. Fluorogenic Membrane Overlays to Enumerate Total and Fecal Escherichia coli and Total Vibrionaceae in Shellfish and Seawater. International Journal of Microbiology. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-507399
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-507399