Transforming Growth Factor β1 Genotypes in Relation to TGFβ1, Interleukin-8, and Tumor Necrosis Factor Alpha in Induced Sputum and Blood in Cystic Fibrosis
Joint Authors
Schmitt-Grohé, S.
Zielen, S.
Eickmeier, O.
Schubert, R.
NGampolo, D.
Lentze, M. J.
Boom, L. v. d.
Schreiner, F.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
High-producer TGFβ1 genotypes are associated with severe lung disease in cystic fibrosis (CF), but studies combining IL-8, TNFα-, and TGFβ1(+genotype) levels and their impact on CF lung disease are scarce.
Aim.
Assessing the relationship between TGFβ1, IL-8, and TNF-α and lung disease in CF in an exacerbation-free interval.
Methods.
Twenty four patients delta F508 homozygous (median age 20.5 y, Shwachman score 75, FEV1(%) 83) and 8 controls (median age 27.5 y) were examined.
TGFβ1 was assessed in serum and induced sputum (IS) by ELISA, for IL-8 and TNF-α by chemiluminescence in IS and whole blood.
Genotyping was performed for TGFβ1 C−509T and T+869C utilizing RFLP.
Results.
TGFβ1 in IS (CF/controls median 76.5/59.1 pg/mL, P<0.074) was higher in CF.
There was a negative correlation between TGFβ1 in serum and lung function (LF) (FEV1 (r=−0.488, P=0.025), MEF 25 (r=−0.425, P=0.055), and VC (r=−0.572, P=0.007)).
Genotypes had no impact on TGFβ1 in IS, serum, and LF.
In IS TGFβ1 correlated with IL-8 (r=0.593, P<0.007) and TNF-α (r=0.536, P<0.018) in patients colonized by bacteria with flagellin.
Conclusion.
TGFβ1 in serum not in IS correlates with LF.
In patients colonized by bacteria with flagellin, TGFβ1 correlates with IL-8 and TNF-α in IS.
American Psychological Association (APA)
Eickmeier, O.& Boom, L. v. d.& Schreiner, F.& Lentze, M. J.& NGampolo, D.& Schubert, R.…[et al.]. 2013. Transforming Growth Factor β1 Genotypes in Relation to TGFβ1, Interleukin-8, and Tumor Necrosis Factor Alpha in Induced Sputum and Blood in Cystic Fibrosis. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-507567
Modern Language Association (MLA)
Eickmeier, O.…[et al.]. Transforming Growth Factor β1 Genotypes in Relation to TGFβ1, Interleukin-8, and Tumor Necrosis Factor Alpha in Induced Sputum and Blood in Cystic Fibrosis. Mediators of Inflammation No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-507567
American Medical Association (AMA)
Eickmeier, O.& Boom, L. v. d.& Schreiner, F.& Lentze, M. J.& NGampolo, D.& Schubert, R.…[et al.]. Transforming Growth Factor β1 Genotypes in Relation to TGFβ1, Interleukin-8, and Tumor Necrosis Factor Alpha in Induced Sputum and Blood in Cystic Fibrosis. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-507567
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-507567