Perfluorochemical Liquid-Adenovirus Suspensions Enhance Gene Delivery to the Distal Lung
Joint Authors
Kazzaz, Jeffrey A.
Wolfson, Marla R.
Koo, Hshi-chi
Malone, Daniel J.
Strayer, David S.
Strayer, Marlene S.
Davis, Jonathan M.
Wu, Jichuan
Shaffer, Thomas H.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-18
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
We compared lung delivery methods of recombinant adenovirus (rAd): (1) rAd suspended in saline, (2) rAd suspended in saline followed by a pulse-chase of a perfluorochemical (PFC) liquid mixture, and (3) a PFC-rAd suspension.
Cell uptake, distribution, and temporal expression of rAd were examined using A549 cells, a murine model using luciferase bioluminescence, and histological analyses.
Relative to saline, a 4X increase in transduction efficiency was observed in A549 cells exposed to PFC-rAd for 2–4 h.
rAd transgene expression was improved in alveolar epithelial cells, and the level and distribution of luciferase expression when delivered in PFC-rAd suspensions consistently peaked at 24 h.
These results demonstrate that PFC-rAd suspensions improve distribution and enhance rAd-mediated gene expression which has important implications in improving lung function by gene therapy.
American Psychological Association (APA)
Kazzaz, Jeffrey A.& Strayer, Marlene S.& Wu, Jichuan& Malone, Daniel J.& Koo, Hshi-chi& Shaffer, Thomas H.…[et al.]. 2011. Perfluorochemical Liquid-Adenovirus Suspensions Enhance Gene Delivery to the Distal Lung. Pulmonary Medicine،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-508025
Modern Language Association (MLA)
Kazzaz, Jeffrey A.…[et al.]. Perfluorochemical Liquid-Adenovirus Suspensions Enhance Gene Delivery to the Distal Lung. Pulmonary Medicine No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-508025
American Medical Association (AMA)
Kazzaz, Jeffrey A.& Strayer, Marlene S.& Wu, Jichuan& Malone, Daniel J.& Koo, Hshi-chi& Shaffer, Thomas H.…[et al.]. Perfluorochemical Liquid-Adenovirus Suspensions Enhance Gene Delivery to the Distal Lung. Pulmonary Medicine. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-508025
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508025