A Phenylbutenoid Dimer, cis-3-(3′,4′-Dimethoxyphenyl)-4-[(E)-3′′′,4′′′-Dimethoxystyryl] Cyclohex-1-ene, Exhibits Apoptogenic Properties in T-Acute Lymphoblastic Leukemia Cells via Induction of p53-Independent Mitochondrial Signalling Pathway
Joint Authors
Ng, Kuan Beng
Anasamy, Theebaa
Abdel Wahab, Siddig Ibrahim
Azid, Mohd Zulkhairi
Mohan, Syam
Sukari, Mohd Aspollah
Sulaiman Rahman, Heshu
Andas, A. Reenaa Joys
Hadi, A. Hamid A.
Muhammad Nadzri, Nabilah
Kamalidehghan, Behnam
Abdul, Ahmad Bustamam
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-28
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
The current study was designed to evaluate the in vitro cytotoxicity effect of a phenylbutenoid dimer, cis-3-(3′,4′-dimethoxyphenyl)-4-[(E)-3‴,4‴-dimethoxystyryl]cyclohex-1-ene (ZC-B11) isolated from the rhizome of Zingiber cassumunar on various cancer cell line, and normal human blood mononuclear cells, and to further investigate the involvement of apoptosis-related proteins that leads, to the probable pathway in which apoptosis is triggered.
Cytotoxicity test using MTT assay showed selective inhibition of ZC-B11 towards T-acute lymphoblastic leukemia cells, CEMss, with an IC50 value of 7.11±0.240 μg/mL, which did not reveal cytotoxic effects towards normal human blood mononuclear cells (IC50 > 50 μg/mL).
Morphology assessments demonstrated distinctive morphological changes corresponding to a typical apoptosis.
ZC-B11 also arrested cell cycle progression at S phase and causes DNA fragmentation in CEMss cells.
Decline of mitochondrial membrane potential was also determined qualitatively.
In the apoptosis-related protein determination, ZC-B11 was found to significantly upregulate Bax, caspase 3/7, caspase 9, cytochrome c, and SMAC and downregulate Bcl-2, HSP70, and XIAP, but did not affect caspase 8, p53, and BID.
These results demonstrated for the first time the apoptogenic property of ZC-B11 on CEMss cell line, leading to the programmed cell death via intrinsic mitochondrial pathway of apoptosis induction.
American Psychological Association (APA)
Anasamy, Theebaa& Abdul, Ahmad Bustamam& Sukari, Mohd Aspollah& Abdel Wahab, Siddig Ibrahim& Mohan, Syam& Kamalidehghan, Behnam…[et al.]. 2013. A Phenylbutenoid Dimer, cis-3-(3′,4′-Dimethoxyphenyl)-4-[(E)-3′′′,4′′′-Dimethoxystyryl] Cyclohex-1-ene, Exhibits Apoptogenic Properties in T-Acute Lymphoblastic Leukemia Cells via Induction of p53-Independent Mitochondrial Signalling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-509865
Modern Language Association (MLA)
Anasamy, Theebaa…[et al.]. A Phenylbutenoid Dimer, cis-3-(3′,4′-Dimethoxyphenyl)-4-[(E)-3′′′,4′′′-Dimethoxystyryl] Cyclohex-1-ene, Exhibits Apoptogenic Properties in T-Acute Lymphoblastic Leukemia Cells via Induction of p53-Independent Mitochondrial Signalling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-14.
https://search.emarefa.net/detail/BIM-509865
American Medical Association (AMA)
Anasamy, Theebaa& Abdul, Ahmad Bustamam& Sukari, Mohd Aspollah& Abdel Wahab, Siddig Ibrahim& Mohan, Syam& Kamalidehghan, Behnam…[et al.]. A Phenylbutenoid Dimer, cis-3-(3′,4′-Dimethoxyphenyl)-4-[(E)-3′′′,4′′′-Dimethoxystyryl] Cyclohex-1-ene, Exhibits Apoptogenic Properties in T-Acute Lymphoblastic Leukemia Cells via Induction of p53-Independent Mitochondrial Signalling Pathway. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-509865
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-509865