VEGF Is Involved in the Increase of Dermal Microvascular Permeability Induced by Tryptase
Joint Authors
Wang, Li
Yin, Lianhua
Zhang, Qingyong
Wang, Xinhong
Li, Xiaobo
Xu, Yali
Bai, Qianming
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Tryptases are predominantly mast cell-specific serine proteases with pleiotropic biological activities and play a critical role in skin allergic reactions, which are manifested with rapid edema and increases of vascular permeability.
The exact mechanisms of mast cell tryptase promoting vascular permeability, however, are unclear and, therefore, we investigated the effect and mechanism of tryptase or human mast cells (HMC-1) supernatant on the permeability of human dermal microvascular endothelial cells (HDMECs).
Both tryptase and HMC-1 supernatant increased permeability of HDMECs significantly, which was resisted by tryptase inhibitor APC366 and partially reversed by anti-VEGF antibody and SU5614 (catalytic inhibitor of VEGFR).
Furthermore, addition of tryptase to HDMECs caused a significant increase of mRNA and protein levels of VEGF and its receptors (Flt-1 and Flk-1) by Real-time RT-PCR and Western blot, respectively.
These results strongly suggest an important role of VEGF on the permeability enhancement induced by tryptase, which may lead to novel means of controlling allergic reaction in skin.
American Psychological Association (APA)
Bai, Qianming& Li, Xiaobo& Wang, Xinhong& Xu, Yali& Wang, Li& Zhang, Qingyong…[et al.]. 2012. VEGF Is Involved in the Increase of Dermal Microvascular Permeability Induced by Tryptase. ISRN Dermatology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-510013
Modern Language Association (MLA)
Bai, Qianming…[et al.]. VEGF Is Involved in the Increase of Dermal Microvascular Permeability Induced by Tryptase. ISRN Dermatology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-510013
American Medical Association (AMA)
Bai, Qianming& Li, Xiaobo& Wang, Xinhong& Xu, Yali& Wang, Li& Zhang, Qingyong…[et al.]. VEGF Is Involved in the Increase of Dermal Microvascular Permeability Induced by Tryptase. ISRN Dermatology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-510013
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-510013