Posttranslational Nitration of Tyrosine Residues Modulates Glutamate Transmission and Contributes to N-Methyl-D-aspartate-Mediated Thermal Hyperalgesia

Joint Authors

Salvemini, Daniela
Dagostino, Concetta
Lauro, Filomena
Ilari, Sara
Mollace, Vincenzo
Palma, Ernesto
Bardhi, Erlisa
Muscoli, Carolina
Gliozzi, Micaela

Source

Mediators of Inflammation

Issue

Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2013-06-20

Country of Publication

Egypt

No. of Pages

12

Main Subjects

Diseases

Abstract EN

Activation of the N-methyl-D-aspartate receptor (NMDAR) is fundamental in the development of hyperalgesia.

Overactivation of this receptor releases superoxide and nitric oxide that, in turn, forms peroxynitrite (PN).

All of these events have been linked to neurotoxicity.

The receptors and enzymes involved in the handling of glutamate pathway—specifically NMDARs, glutamate transporter, and glutamine synthase (GS)—have key tyrosine residues which are targets of the nitration process causing subsequent function modification.

Our results demonstrate that the thermal hyperalgesia induced by intrathecal administration of NMDA is associated with spinal nitration of GluN1 and GluN2B receptor subunits, GS, that normally convert glutamate into nontoxic glutamine, and glutamate transporter GLT1.

Intrathecal injection of PN decomposition catalyst FeTM-4-PyP5+ prevents nitration and overall inhibits NMDA-mediated thermal hyperalgesia.

Our study supports the hypothesis that nitration of key proteins involved in the regulation of glutamate transmission is a crucial pathway used by PN to mediate the development and maintenance of NMDA-mediated thermal hyperalgesia.

The broader implication of our findings reinforces the notion that free radicals may contribute to various forms of pain events and the importance of the development of new pharmacological tool that can modulate the glutamate transmission without blocking its actions directly.

American Psychological Association (APA)

Muscoli, Carolina& Dagostino, Concetta& Ilari, Sara& Lauro, Filomena& Gliozzi, Micaela& Bardhi, Erlisa…[et al.]. 2013. Posttranslational Nitration of Tyrosine Residues Modulates Glutamate Transmission and Contributes to N-Methyl-D-aspartate-Mediated Thermal Hyperalgesia. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-510794

Modern Language Association (MLA)

Muscoli, Carolina…[et al.]. Posttranslational Nitration of Tyrosine Residues Modulates Glutamate Transmission and Contributes to N-Methyl-D-aspartate-Mediated Thermal Hyperalgesia. Mediators of Inflammation No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-510794

American Medical Association (AMA)

Muscoli, Carolina& Dagostino, Concetta& Ilari, Sara& Lauro, Filomena& Gliozzi, Micaela& Bardhi, Erlisa…[et al.]. Posttranslational Nitration of Tyrosine Residues Modulates Glutamate Transmission and Contributes to N-Methyl-D-aspartate-Mediated Thermal Hyperalgesia. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-510794

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-510794