Novel Biphasic Role of Resolvin D1 on Expression of Cyclooxygenase-2 in Lipopolysaccharide-Stimulated Lung Fibroblasts Is Partly through PI3KAKT and ERK2 Pathways

Abstract EN

Fibroblasts, far frombeing merely bystander cells, are known to play a specific role in inflammation resolution after an acute injury.

As the endogenous “braking signal,” resolvins possess potent anti-inflammatory and proresolution actions.

We demonstrated that the expression of COX-2 protein was significantly peaked initially at 6 hours but then also at 48 hours after LPS stimulation in lung fibroblasts.

PGE2 levels also peaked at 6 hours, and PGD2 levels were increased and peaked at 48 hours.

However, no significant change in the protein expression of COX-1 was observed after treatment with LPS in lung fibroblasts.

Exogenous resolvin D1 inhibited the first peak of COX-2 expression as well as the production of PGE2 induced by LPS.

In contrast, exogenous resolvin D1 increased the second peak of COX-2 expression as well as the production of PGD2 induced by LPS.

In addition, resolvin D1 inhibited COX-2 expression at 6 hours, which was partly through PI3K/AKT and ERK2 signalling pathways.