Expression and Purification of Z Protein from Junín Virus
Joint Authors
Tortorici, M. A.
Iserte, Javier Alonso
Romano, F. B.
Rota, R. P.
Ghiringhelli, Pablo Daniel
Pilloff, M. G.
Lozano, Mario Enrique
Goñi, Sandra Elizabeth
Bilen, M. F.
Stephan, Betina Ines
Borio, C. S.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-29
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Arenaviridae comprises 23 recognized virus species with a bipartite ssRNA genome and an ambisense coding strategy.
The virions are enveloped and include nonequimolar amounts of each genomic RNA species, designated L and S, coding for four ORFs (N, GPC, L, and Z).
The arenavirus Junín (JUNV) is the etiological agent of Argentine Hemorrhagic Fever, an acute disease with high mortality rate.
It has been proposed that Z is the functional counterpart of the matrix proteins found in other negative-stranded enveloped RNA viruses.
Here we report the optimized expression of a synthetic gene of Z protein, using three expression systems (two bacterial and a baculoviral one).
One of these recombinant proteins was used to generate antibodies.
A bioinformatic analysis was made where Z was subdivided into three domains.
The data presented contributes methodologies for Z recombinant production and provides the basis for the development of new experiments to test its function.
American Psychological Association (APA)
Goñi, Sandra Elizabeth& Borio, C. S.& Romano, F. B.& Rota, R. P.& Pilloff, M. G.& Iserte, Javier Alonso…[et al.]. 2010. Expression and Purification of Z Protein from Junín Virus. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-14.
https://search.emarefa.net/detail/BIM-512356
Modern Language Association (MLA)
Goñi, Sandra Elizabeth…[et al.]. Expression and Purification of Z Protein from Junín Virus. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-14.
https://search.emarefa.net/detail/BIM-512356
American Medical Association (AMA)
Goñi, Sandra Elizabeth& Borio, C. S.& Romano, F. B.& Rota, R. P.& Pilloff, M. G.& Iserte, Javier Alonso…[et al.]. Expression and Purification of Z Protein from Junín Virus. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-14.
https://search.emarefa.net/detail/BIM-512356
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512356