تأثير كلايكول متعدد الأثيلين (PEG)‎ في تضاعف الأفرع لبعض أصول الحمضيات خارج الجسم الحي

Abstract EN

An experiment was conducted to study the effect of four levels of PEG on in vitro shoots proliferation of five citrus rootstocks (Sour orange, Cleopatra mandarin, Troyer citrange, Volkamer lemon and Swingle Citrumelo) according to the effects ofpoly ethylene glycol (PEG) on their morphological characters at multiplication stage.

Double-node micro cuttings were excised from in vitro proliferating shoots cultured in proliferation medium (MS + 2 mg BA + 0.1 mg NAA / L + 30 mg adenine sulphate) supplemented with four PEG levels (0, 1, 2 and 3 %) for 4 weeks.

There was a significant reduction in Survival percentage, shoots number, shoots length, number of leaves per shoot, fresh and dry weight, with increasing PEG level in multiple shoot culture medium.

Lowest values were obtained at 3 % PEG (44.64 %, 2.1.4 shoots, 1.4.0 cm, 0.201 leaf / shoot, 041.0 mg and 66.6 mg respectively), whereas, those obtained from shoots ofcontrol (0 % PEG) revealed (100 %, 0.044 shoots, 0.401 cm, 4.120 leaf / shoot, 621.6 mg and 001.4 mg respectively).

Rootstocks was showed different behavior under water stress induced by addition PEG to the medium culture.

Troyer Citrange was superior to other rootstocks in most morphological characters, but there was no significant differences between Troyer Citrange and Swingle Citrumelo in shoots number, shoot dry weight.