The antimicrobial activity of bacteriocin from pseudomonas fluorescens against pathogenic bacteria

Other Title(s)

الفعالية التثبيطية لبكتريا Pseudomonas Fluorescens على نمو البكتريا المرضية

Joint Authors

Kazim, Shuruq Rayyis
Ahmad, Kifah Jasim
Isam, Maysa A.

Source

Basrah Journal of Science

Issue

Vol. 32, Issue 2B (31 Dec. 2014), pp.206-213, 8 p.

Publisher

University of Basrah College of Science

Publication Date

2014-12-31

Country of Publication

Iraq

No. of Pages

8

Main Subjects

Biology

Topics

Abstract AR

اختبرت قابلية أربع عزلات محلية من بكتريا الـ Pseudomonas f luorescens على إنتاج البكتريوسين بطريقة قطع الأكار agar block و استخدمت العزلات السريرية Aeromonas hydrophilia و Escherichiacoli coli و Klebsiella pneumoniae و Pseudomonas aeruginosa, Salmonella typhi و Staphylococcus aureus و Streptococcus faecalis و Vibrio cholerae (enaba) كعزلات دالة حساسة.

كانت العزلات الأربعة منتجة للبكتريوسين و بمدى تثبيط واسع (8-12 مليمتر) لنمو البكتريا الموجبة و السالبة لصبغة كرام.

أظهرت النتائج أن العزلة Pseudomonas fluorescens P1 قد ثبطت النمو البكتيري بمدى (8-18 ملمتر) عدا بكتريا Vibrio cholera.

بينما العزلة Pseudomonas fluorescens P2 أنتجت تثبيطا للعزلات الحساسة بمدى تراوح بين (7-21 ملميتر) Vibrio cholera و Streptococcus faecalis.

أما العزلة P3 فكان تأثيرها التثبيطي لنمو العزلات الحساسة بين (10-16 ملمتر) عدا Streptococcus faecalis و Klebsiella pneumoniae.

كما أظهرت العزلة الرابعة تأثيرا تثبيطيا للنمو تراوح بين (8-16 ملمتر).

كانت بكتريا الـ Escherichia coli هي الأكثر تأثرا بالبكتريوسين المنتج من Pseudomonas fluorescens تبعتها بكتيريا Staphylococcus Salmonella aureus و Streptococcus faecalis و Klebsiella pneumoniae و Vibrio cholera (enaba) ثم Pseudomonas aeruginosa على التوالي.

Abstract EN

In this study four isolates of Pseudomonas fluorescens were tested for bacteriocin production by the agar block method.

Clinical bacterial isolates like; Aeromonas hydrophilia, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus aureus, Streptococcus faecalis and Vibrio cholerae(enaba) were used as indicator isolates.

All the isolates were positive as produce with a wide range effect on gram positive and negative bacterial growth, with diameter (8-21) mm.

The results showed that Pseudomonas fluorescens P1 inhibit the bacterial growth of the tested isolates with a range of inhibition zone (8-18) mm.

except Vibrio cholera (enaba) while P2 inhibit the bacterial growth of the tested isolates and the range of inhibition zone was (7-21) mm.

Except Vibrio cholera (enaba) and Streptococcus faecalis.

On the other hand P3 inhibit the growth of the isolates with a zone of inhibition between (10-16) mm.

except Streptococcus faecalis and Klebsiella pneumoniae.

The isolate P4 inhibited the growth of all the tested isolates with a range of inhibition zone between (8-19) mm.

Escherichia coli was the most affected bacteria by bacteriocin of P.

fluorescens, followed by Salmonella typhi, Staphylococcus aureus, Streptococcus faecalis, Klebsiella pneumoniae, Vibrio cholera (enaba) and Pseudomonas aeruginosa.

American Psychological Association (APA)

Kazim, Shuruq Rayyis& Ahmad, Kifah Jasim& Isam, Maysa A.. 2014. The antimicrobial activity of bacteriocin from pseudomonas fluorescens against pathogenic bacteria. Basrah Journal of Science،Vol. 32, no. 2B, pp.206-213.
https://search.emarefa.net/detail/BIM-542694

Modern Language Association (MLA)

Kazim, Shuruq Rayyis…[et al.]. The antimicrobial activity of bacteriocin from pseudomonas fluorescens against pathogenic bacteria. Basrah Journal of Science Vol. 32, no. 2B (2014), pp.206-213.
https://search.emarefa.net/detail/BIM-542694

American Medical Association (AMA)

Kazim, Shuruq Rayyis& Ahmad, Kifah Jasim& Isam, Maysa A.. The antimicrobial activity of bacteriocin from pseudomonas fluorescens against pathogenic bacteria. Basrah Journal of Science. 2014. Vol. 32, no. 2B, pp.206-213.
https://search.emarefa.net/detail/BIM-542694

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 211-212

Record ID

BIM-542694