Molecular detection of ALS1 virulence gene of Candida albicans isolated from groups of Iraqi patients
Joint Authors
al-Ahmar, Sayf Dawud
Ali, Aishah Sabir
al-Atraqji, Azhar A. F.
Source
Journal of the Faculty of Medicine Baghdad
Issue
Vol. 57, Issue 1 (31 Mar. 2015), pp.79-83, 5 p.
Publisher
University of Baghdad Faculty of Medicine
Publication Date
2015-03-31
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Topics
Abstract EN
Background : Candida albicans is the principal fungal infectious agent in human infection.
Adhesion is thought to be an essential step for colonization and establishment of Candida infections.
Objectives : Identification and comparison of ALS1 virulence gene of adhesion family among different isolates of Candida albicans by PCR.
Patients and methods : One hundred eight samples were collected from different group of Iraqi patients.
All samples were culture on Sabouraud′s agar, CHROMagar for identification while API Candida kit confirmatory test and extracted DNA was done for just Candida albicans isolates, detected the ALS1 gene, extracted RNA for synthesis of cDNA and detected of gene and compare between isolates.
Result : C.
albians isolated from each of vaginal and oral swabs expressed the stronger gene that mean these isolates were the most virulent once.
The two isolates from each of urine sample and nail clip which expressed only one strong fluorescence represented by ALS1 gene.
C.
albicans isolated from cutaneous scrap sample showed the only one moderate fluorescence represented by ALS1 gene.
Conclusion : C.
albians isolated from each of vaginal and oral swabs expressed the stronger gene that’s mean these isolates were the most virulent once.
American Psychological Association (APA)
Ali, Aishah Sabir& al-Atraqji, Azhar A. F.& al-Ahmar, Sayf Dawud. 2015. Molecular detection of ALS1 virulence gene of Candida albicans isolated from groups of Iraqi patients. Journal of the Faculty of Medicine Baghdad،Vol. 57, no. 1, pp.79-83.
https://search.emarefa.net/detail/BIM-546584
Modern Language Association (MLA)
Ali, Aishah Sabir…[et al.]. Molecular detection of ALS1 virulence gene of Candida albicans isolated from groups of Iraqi patients. Journal of the Faculty of Medicine Baghdad Vol. 57, no. 1 (2015), pp.79-83.
https://search.emarefa.net/detail/BIM-546584
American Medical Association (AMA)
Ali, Aishah Sabir& al-Atraqji, Azhar A. F.& al-Ahmar, Sayf Dawud. Molecular detection of ALS1 virulence gene of Candida albicans isolated from groups of Iraqi patients. Journal of the Faculty of Medicine Baghdad. 2015. Vol. 57, no. 1, pp.79-83.
https://search.emarefa.net/detail/BIM-546584
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 82-83
Record ID
BIM-546584