تأثير الباكلوبيوترازول في المحتوى الكاربوهيدراتي و البروتيني لكالس نخيل التمر Phoenix dactylifera L. صنف نيرسي

Abstract EN

The present study has been performed at the labs .

Of Agricultural college /Basra University alluring the period between 2/11/2013 and 2/3/2014.

A primary date palm Callus of Nersi cv.

has been used and Treated with Paclobutrazol and Casein to evaluated their effect on carbohydrate and protein contents of embryogeneic callus after eight weeks of culturing .

The primary callus has been cultured at the average of 100 mg in the cultural medium of MS , supplemented with NAA at concentration of 10 mg/L , cytokinin 2ip at concentration of 2mg/L and activated Charcol at concentration of 2g/L .

Fist Cultural medium supplemented with different concentrations of paclobutrazol , which were 0, 0.3, 0.5, 0.7, 0.9 and 1.0 mg/L, while the concentration of 0 , 200 , 400 , 600 , 800 and 1000 mg/L were used with Casein treatment .

Subcultural procedure has been conduit of on the same above-mentioned media at month interval , up to 4 months .

Results of study explained that the treatment of paclobutrazol (PAC) at conc.

of 1.0 mg/L was the most effective treatment and led to a significant increase in Carbohydrate content of embryogeneic Callus, followed by the treatment of PAC at 0.9 mg/L .

Hence , both PAC at concentration of 0.5 and 0.7 mg/L led to an increase in the total protein Content Noteworthy , all concentration of Casein (CH) led to a positive and Significant increase for both content of Carbohydrate and Protein , especially at concentration 1000 mg/L .