إنتاج مضاد الاستحلاب الحيوي بواسطة عزلات بكتيرية معزولة من الترب المحلية

Abstract EN

The purpose of this research was to isolate local bacterial have the effectiveness of de-emulsification in liquid emulsion.

Twenty local isolates of actinomycetes were isolated from soil contaminated with oil waste using mineral liquid medium at initial pH of 7 in the presence of oil waste (1%) as a source of carbon and incubated at 40 °C for 5 days .

The primary screening of isolates was tested to de-emulsifying ability.Results indicate that the isolates ST3 , ST8 and ST15 were selected for theirhigh ability to deemulsification.

Secondary screening of selected isolates was estimated to deemulsification using the spore suspension .

Results indicate that the ST8 isolate showed the highest deemulsification activity, subsequently was identified and designated as Nocardia sp..

Studied the effects of both the spores number and period of exposure on the effectiveness of de-emulsification.

The results showed that the 0.5 ml of spore suspension (107 spore/ml) gave the best effective way to de-emulsifying, while 30 min.

the best period appropriate to de-emulsifying.