Rosemary leaves aqueous extract for protection against acute doxorubicin-induced cardiotoxicity in mice
Joint Authors
al-Mukhtar, Intisar Jawad
al-Sahib, Zaynah Hasan
Jawad, Afra M.
Naji, Hamid
Source
Issue
Vol. 12, Issue 2 (30 Jun. 2015), pp.385-397, 13 p.
Publisher
University of Babylon College of Medicine
Publication Date
2015-06-30
Country of Publication
Iraq
No. of Pages
13
Main Subjects
Topics
Abstract EN
Doxorubicin (DOX) is a cancer chemotherapy widely used to treat a many types of human malignancies, DOX is potent anthracycline antibiotic.
As a complicating effect of DOX cardiotoxicity has long been recognized ; To explain this cardiotoxicity there are several hypotheses and the most thoroughly investigated one is free radical hypothesis.
Our study was designed to investigate if the aqueous extract of rosemary leaves has a protective effect against cardiotoxicity induced by DOX in mice.
Twenty eight male Swiss Albino mice were randomly divided into four groups including group1 (negative control), treated with distill water (D.
W), group 2 (positive control), treated with 15 mg / kg DOX as a single intraperitoneal (i.p) injection, groups 3 and 4 received 15mg / kg and 30mg / kg respectively of the aqueous extract of Rosmarinus officinalis leaves (ROE) orally (p.o), once daily for 2 weeks, then injected i.p with 15 mg / kg DOX.
Two days after DOX or D.W (in control group) injection, animals in all groups were scarified and the levels of the cardiac biomarkers including serum creatine kinase (CK-MB) and serum lactate dehydrogenase (LDH) were measured.
Also the cardiac histopathological sections were prepared, stained by hematoxylin and eosin stains and examind under light microscope.
The administration of 15mg / kg DOX caused cardiomyopathy which was manifested by extremely significant elevation (p < 0.
001) in serum CK-MB and LDH levels.
In addition, cardiac histopathological sections showed moderate cytoplasm vacuolization and inflammatory cells infiltrate with vascular congestion.
Oral administration of 30mg / kg ROE for 2 weeks prior to DOX provided significant protection which was evidenced by extremely significant reduction (p < 0.
001) in the levels of CK-MB and LDH.
Moreover, histopathological sections revealed only mild cytoplasm vacuolization, infiltration of inflammatory cells and vascular congestion in comparison to DOX positive control group (p < 0.
01).
Whereas oral administration of 15mg / kg ROE for 2 weeks prior to DOX showed no significant protection neither in CK-MB and LDH levels, nor in the histopathological sections.
Administration of 30mg / kg ROE protect against DOX-induced cardiotoxicity.
This might serve as novel adjuvant therapy with DOX.
American Psychological Association (APA)
al-Mukhtar, Intisar Jawad& al-Sahib, Zaynah Hasan& Jawad, Afra M.& Naji, Hamid. 2015. Rosemary leaves aqueous extract for protection against acute doxorubicin-induced cardiotoxicity in mice. Medical Journal of Babylon،Vol. 12, no. 2, pp.385-397.
https://search.emarefa.net/detail/BIM-593580
Modern Language Association (MLA)
al-Mukhtar, Intisar Jawad…[et al.]. Rosemary leaves aqueous extract for protection against acute doxorubicin-induced cardiotoxicity in mice. Medical Journal of Babylon Vol. 12, no. 2 (2015), pp.385-397.
https://search.emarefa.net/detail/BIM-593580
American Medical Association (AMA)
al-Mukhtar, Intisar Jawad& al-Sahib, Zaynah Hasan& Jawad, Afra M.& Naji, Hamid. Rosemary leaves aqueous extract for protection against acute doxorubicin-induced cardiotoxicity in mice. Medical Journal of Babylon. 2015. Vol. 12, no. 2, pp.385-397.
https://search.emarefa.net/detail/BIM-593580
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 393-397
Record ID
BIM-593580