تقييم مستضدات السائل العداري للكشف عن أضداد الكيسات العدارية الغنمية باستخدام الأليزا غير المباشرة

Abstract EN

Hydatidosis is a dangerous zoonosis with worldwide distribution and causes heavy losses in sheep.

Forty positive serum samples were collected from sheep infected with hydatidosis, and 24 serum samples from uninfected sheep, and additional 11 samples from sheep with Cysticercosis tenuicollis were collected Antibodies against Hydatid cysts were detected with indirect ELISA method using partially separated antigens of hepatic Hydatid fluid.

The study aimed to evaluate the sensitivity and specificity of the indirect ELISA method using hepatic hydatid fluid antigens in order to improve the sero-diagnostic method for use in sero-epidemiological survey of the disease in sheep.

The results showed that the sensitivity and the specificity of the indirect ELISA using hepatic hydatid fluid antigen were found to be 87.5%, 66.7%, respectively.

Cross-reactions with Cysticercosis tenuicollis were found to be 36.36%.

It is concluded that there is a possibility to use hydatid fluid antigens to detect hydatid cyst antibodies using indirect ELISA in Awassi sheep, but the specificity of the test is still low.

Therefore, it is necessary to search for high antigenicity proteins with low cross reaction.