تحديد المستضدات الرئيسة للسائل العداري الكبدي و الرئوي عند الأغنام العواس في سورية

Abstract EN

Hydatidosis is a dangerous parasitic zonoosis caused by larval stage of Echinococcus granulosus, with worldwide distribution and important economic impact in sheep Specific diagnostic antigens of Hydatidosis were isolated from fluids, liver and lung Hydatid cysts that collected from slaughtered sheep in Hama and countryside Damascus to determine the protein subunits that posse’s antigenic activity.

The antigenic proteins were purified by passing through the sephadex G-100 in chromatography column, after precipitation with ammonium sulfate, centrifugation, and dialysis against distilled and deionized watter, to obtain the antigenic active proteins and disposal the unimportant proteins, and separating it in SDS- PAGE and detection it by western blot.

The result showed that the subunits of hepatic hydatid fluid of 54, 38 and 27 KDa bands reacted with reference positive sera in the immunoblotting technique on nitrocellulose membranes, while the 67 and 16 KDa bands didn’t react.

On the other hand, the subunits of lung Hydatid fluid in the weight 22, 28, 38 and 54 KDa reacted with reference sera from sheep with Cystic Echinococcosis, which don,t react with both bands in weight 12 and 67 KDa.

It can be concluded, it is possible to use liver and lungs Hydatid fluid subunits having antigenic activity as specific antigens in diagnostic tes