تنميط جزيئي لسلالات الليشمانِية الجلدية المعزولة من مرضى في اليمن بطريقة التفاعل السلسلي للبوليميراز PCR

Abstract EN

Leishmaniasis is one of the major parasitic diseases in under development country, widespread in many of the Middle East regions, Saudi Arabia, Jordan, Syria, and Yemen.

The disease is present in three clinical forms: cutaneous, mucocutaneous and visceral leishmaniasis.

A set of methods for diagnosis and identifying the type of parasite have been developed in order to give appropriate treatment.

These methods include isoenzyme analysis, serological, immunological methods and DNA hybridization.

The polymerase chain reaction (PCR), using genomic or kinetoplastid DNA; provides an excellent tool for diagnosis and characterization of leishmania species.

In this study we worked on samples isolated from Yemeni patients affected with Cutaneous Leishmaniasis.

The diagnosis was based on direct microscopic observation by skin slit smears taken from the edge of the Lesions.

We identified leishmania species by polymerase chain reaction (PCR).

Our results showed cutaneous Leishmaniasis was caused by L.tropica.