In vitro anti-cancer activities of Job’s tears (Coix lachryma-jobi Linn.) extracts on human colon adenocarcinoma
Joint Authors
Manosroi, Aranya
Sainakham, Mathukorn
Chankhampan, Charinya
Manosroi, Worapaka
Manosro, Jiradej
Source
Saudi Journal of Biological Sciences
Issue
Vol. 23, Issue 2 (31 Mar. 2016), pp.248-256, 9 p.
Publisher
Publication Date
2016-03-31
Country of Publication
Saudi Arabia
No. of Pages
9
Main Subjects
Topics
Abstract EN
The whole seed (W), endosperm (E) and hull (H) of five cultivars of Job’s tears (Coix lachryma-jobi Linn.
var.
ma-yuen Stapf) including Thai Black Phayao, Thai Black Loei, Laos Black Loei, Laos White Loei and Laos Black Luang Phra Bang were processed before solvent extraction by non-cooking, roasting, boiling and steaming Each part of the Job’s tears was extracted by the cold and hot process by refluxing with methanol and hexane.
The total of 330 extracts included 150 methanol extracts and 180 hexane extracts were investigated for anti-proliferative activity on human colon adenocarcinoma cell line (HT-29) by the sulforhodamine B (SRB) assay.
The extracts which gave high anti-proliferative activity were tested for apoptotic activity by acridine orange and ethidium bromide double staining and anti-oxidative activities including free radical scavenging and lipid peroxidation inhibition activities.
The extract from the hull of Thai Black Loei roasted before extracting by hot methanol (M-HTBL-R2) showed the highest anti-proliferative activity on HT-29 with the IC50 values of 11.61 ± 0.95 lg/ml, while the extract from the non-cooked hull of Thai Black Loei by cold methanol extraction (M-HTBL-N1) gave the highest apoptosis (8.17 ± 1.18%) with no necrosis.
In addition, M-HTBL-R2 and M-HTBL-N1 indicated
American Psychological Association (APA)
Manosroi, Aranya& Sainakham, Mathukorn& Chankhampan, Charinya& Manosroi, Worapaka& Manosro, Jiradej. 2016. In vitro anti-cancer activities of Job’s tears (Coix lachryma-jobi Linn.) extracts on human colon adenocarcinoma. Saudi Journal of Biological Sciences،Vol. 23, no. 2, pp.248-256.
https://search.emarefa.net/detail/BIM-674858
Modern Language Association (MLA)
Manosroi, Aranya…[et al.]. In vitro anti-cancer activities of Job’s tears (Coix lachryma-jobi Linn.) extracts on human colon adenocarcinoma. Saudi Journal of Biological Sciences Vol. 23, no. 2 (2016), pp.248-256.
https://search.emarefa.net/detail/BIM-674858
American Medical Association (AMA)
Manosroi, Aranya& Sainakham, Mathukorn& Chankhampan, Charinya& Manosroi, Worapaka& Manosro, Jiradej. In vitro anti-cancer activities of Job’s tears (Coix lachryma-jobi Linn.) extracts on human colon adenocarcinoma. Saudi Journal of Biological Sciences. 2016. Vol. 23, no. 2, pp.248-256.
https://search.emarefa.net/detail/BIM-674858
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 255-256
Record ID
BIM-674858