تقييم التأثير السمي لمستخلصات بعض الطحالب في خط خلايا سرطان الحنجرة البشري 2-Hep

Abstract EN

In this Study, isolate and identification Three types of algae Scenedesmus obliguus of the belong to Division of green –algae and Anabaena cylindrical and Lyngbya sp.

Of the belong to Division of cyanobacteria from channel at the University of Baghdad.

Use culture medium Chu- 10 for growth of algae on batch culture in the laboratory conditions (25 ˚ c ±2 and light intensity 200 μE/m²/sec the light: dark regime was used 16:8 hrs).

Harvested culture after fourteen days of age farm.

Use methanol 95% to extract active compound from raw dry biomass, Tested the effectiveness of the efficiency of the cell extract toward the cell line (human larynx cancer) Hep-2 from biotechnology center at the University of Alnahrain university and different concentrations (125, 250, 500, 1000, 2000 and 4000 μg / mL) The study showed that the extract of green algae Scenedesmus obliguus better the effectiveness of the extract blue green alga Anabaena cylindrical and Lyngbya sp.

toward the cell line Hep -2 found that the extract has a toxic effect of all concentrations had a higher effect Higher toxic effect of the extract at concentration Senedesmus obliguus 1000 μg / mL by 71.45%.while the toxic effect was higher when the concentration 4000 μg / mL by 70.9% and 71.8%% to blue-green alga extract Anabaena cylindrical and Lyngbya sp.

respectively Did not show inhibitory effect at low concentrations in this study.

These anti Hep-2 agents were partially purified and the pure active compounds proved to have higher activity comparing with the cruds extract about 20% at 200 μg / mLconsentration.