انتشار و إنتاج السم المعوي enterotoxin B و الحساسية للمضادات الحيوية لعزلات جرثومة المكورات العنقودية المعزولة من الإسهال

Abstract EN

One hundred and seventy five samples were collected from diarrhea swabs, twelve isolates (6.7%) were obtained and diagnosed as Staphylococcus aureus.

The production of enterotoxin B were tested by using reverse passive latex agglutination.

Eight isolates (66.7%) had the ability to producing enterotoxin B.

S.

aureus were 100% resistancy were recorded for two antibiotics, ampicillin and penicillin, while in the opposite direction, 100% sensitive to Fusidic acid .

Graded resistant was observed in other antibiotics, include: 66.7% for erythromycin, tetracycline, oxacillin and methicillin.

The results of this study showed the wide spread of enterotoxigenic and multidrug-resistant S.

aureus isolates isolated from diarrhea samples.

The lowest temperature at which SEB production manifested was 20°C in four isolates (SA2,SA4,SA11 and SA12), while the highest temperature at which SEB was produced was 45°C, in three isolates (SA2,SA11 and SA12).

The lowest pH value at which SEB production was still observed was 4 in two isolates ( SA4 and SA12) .

These isolates were able to produce SEB even at pH 10.5, which was the highest pH value among the isolates studied.

SEB is produced within a wide pH range but the influence of temperature is an essential one for the production of this toxin.