Isolation and purification of a cyclooxygenase and 2 from the blood of a patient suffering from rheumatoid arthritis and studying the effect of natural products of the soapwort on the activity of purified enzyme
Other Title(s)
عزل و تنقية أنزيم السايكلوكسي جينيز-2 من دم مريض يعاني التهاب المفاصل الرثوي و دراسة تأثير النواتج الطبيعية لنبات العصلج على فعالية الأنزيم المنقى
Joint Authors
al-Sadun, Muhammad Bahri Hasan
al-Sabawi, Usamah Muyassar Mahmud
Source
Issue
Vol. 13, Issue 1 (31 Mar. 2016), pp.133-145, 13 p.
Publisher
University of Baghdad College of Science for Women
Publication Date
2016-03-31
Country of Publication
Iraq
No. of Pages
13
Main Subjects
Topics
Abstract EN
In this paper to isolate and study the properties of the cyclooxygenase-2 (EC : 1.14.99.1) enzyme in the blood of a patient suffering from rheumatoid arthritis and study the effect of natural products of the Soapwort on the activity of purified enzyme.
The study involves taking 30 ml of blood from an adult woman 40 years old, who suffers from rheumatoid arthritis disease for 13 years.
Serum is separated and subjected to a series of purification processes including: precipitation by ammonium sulfate, filtration by centrifugation radiator, dialysis in presence of ammonium sulfate, separation using the technology of ion exchange, lipholization and then estimating approximate molecular weight of the enzyme using gel filtration technique and sodium dodecyl sulfate (SDS)-page polyacrylamide gel electrophoresis.
The study also includes isolating the natural products of Soapwort plant and study the effect of isolated natural products on the activity of the purified enzyme.
The result of the study indicates that cycloxygenase-2 has an approximate molecular weight of 71.5 kDa and that the extracted oil of the Soapwort has a negative impact on the activity of the enzyme (r = -0.824; P = 0.006), while flavonoids and Saponin have no such impact (r = -0.565; P =0.113; r = -0.634; P = 0.067 respectively).
American Psychological Association (APA)
al-Sadun, Muhammad Bahri Hasan& al-Sabawi, Usamah Muyassar Mahmud. 2016. Isolation and purification of a cyclooxygenase and 2 from the blood of a patient suffering from rheumatoid arthritis and studying the effect of natural products of the soapwort on the activity of purified enzyme. Baghdad Science Journal،Vol. 13, no. 1, pp.133-145.
https://search.emarefa.net/detail/BIM-688206
Modern Language Association (MLA)
al-Sadun, Muhammad Bahri Hasan& al-Sabawi, Usamah Muyassar Mahmud. Isolation and purification of a cyclooxygenase and 2 from the blood of a patient suffering from rheumatoid arthritis and studying the effect of natural products of the soapwort on the activity of purified enzyme. Baghdad Science Journal Vol. 13, no. 1 (2016), pp.133-145.
https://search.emarefa.net/detail/BIM-688206
American Medical Association (AMA)
al-Sadun, Muhammad Bahri Hasan& al-Sabawi, Usamah Muyassar Mahmud. Isolation and purification of a cyclooxygenase and 2 from the blood of a patient suffering from rheumatoid arthritis and studying the effect of natural products of the soapwort on the activity of purified enzyme. Baghdad Science Journal. 2016. Vol. 13, no. 1, pp.133-145.
https://search.emarefa.net/detail/BIM-688206
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 142-144
Record ID
BIM-688206