Phenotypic detection of metallo-β-lactamase in imipenem resistant Pseudomonas aeruginosa in al-Sadr Hospital. Iraq
Author
Source
al-Kufa University Journal for Biology
Issue
Vol. 7, Issue 3 (31 Dec. 2015), pp.55-65, 11 p.
Publisher
University of Kufa Faculty of Science Department of live Sciences
Publication Date
2015-12-31
Country of Publication
Iraq
No. of Pages
11
Main Subjects
Abstract EN
From October 2013 to the end of March 2014, 26 isolates of Pseudomonas aeruginosa were isolated from patients visited Al-Sadr Medical City Hospital suffering from various infections.
These isolates were tested using disk diffusion test for susceptibility of imipenem, where five isolates were resistant to this agent, 3 were moderate, while 18 isolates were susceptible.
The resistant isolates were submitted to Minimum Inhibitory Concentration (MIC) assay using E-test strips (Dilution and Diffusion method).
The MIC values varied from 1-12 µg/ml.
Phenotypic detection for Metallo–β–Lacamases enzymes in resistant isolates was done using two tests, the Double Disk Synergy Test (DDST), and Combined Disk Test (CDT).
The results showed that three isolates were positive to MBL production by (DDST)at (10) mm, and two at (15)mm , only one isolate was positive by the CDTs giving diameter difference (16) mm.
American Psychological Association (APA)
Shubbar, Ibtihal Idris. 2015. Phenotypic detection of metallo-β-lactamase in imipenem resistant Pseudomonas aeruginosa in al-Sadr Hospital. Iraq. al-Kufa University Journal for Biology،Vol. 7, no. 3, pp.55-65.
https://search.emarefa.net/detail/BIM-691951
Modern Language Association (MLA)
Shubbar, Ibtihal Idris. Phenotypic detection of metallo-β-lactamase in imipenem resistant Pseudomonas aeruginosa in al-Sadr Hospital. Iraq. al-Kufa University Journal for Biology Vol. 7, no. 3 (2015), pp.55-65.
https://search.emarefa.net/detail/BIM-691951
American Medical Association (AMA)
Shubbar, Ibtihal Idris. Phenotypic detection of metallo-β-lactamase in imipenem resistant Pseudomonas aeruginosa in al-Sadr Hospital. Iraq. al-Kufa University Journal for Biology. 2015. Vol. 7, no. 3, pp.55-65.
https://search.emarefa.net/detail/BIM-691951
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 64-65
Record ID
BIM-691951