التنوع الوراثي لعزلات الفطر Fusarium oxysporum f. sp cucumerinum المعزول من من نباتات الخيار باستعمال تقنية التضخيم العشوائي متعدد الأشكال لقطع DNA

Abstract EN

This study was conducted at the Molecular Genetics laboratory, college of agriculture, university of Basrah (UOB) which is aimed to detect the genetic biodiversity by using random amplified polymorphism DNA markers (PCR-RAPD) between the isolates of Fusarium oxysporum f.sp cucumerinum isolated from infected plants stems cucumber which has been obtained from the different regions of Basrah province.

Stages of the work included the extraction DNA from isolates F.

o.

f.sp cucumerinum depending on the CTAB Buffer method and using 8 primers imported from Operon company, U.S.A.

to measure their efficiency.

The results of DNA extraction, which extracted by using CTAB Buffer method from all isolates of F.

o.

f.sp cucumerinum it was good quality, since it has proved its efficiency in the following tests.

RAPD primers gave good results through the successful amplification of DNA for all F.

o.f.sp cucumerinum isolated from the infected plants stems cucumber and each primer succeeded in distinguishing between isolates, as appeared polymorphic and monomorphic bands.

The primer OPB-08 Showed highly efficient in total number of bands (35) and less primers efficiency is OPB-14 gave the lowest number of bands (17).

The number of polymorphic fragments per primer was 18.5.

The size of amplification DNA fragments generated with the RAPD primers ranged from 0.1 to 1.6 kb.

The results of this study indicated that PCR-based technique could be used to differentiate the F.

o.f.sp cucumerinum isolates from cucumber plants.