دراسة تأثير العمر او عدم وجود الجسم الأصفر في عدد البويضات المستحصلة و المنضجة و على هرمونات السائل الجريبي في الجاموس

Abstract EN

This study aimed to obtain the oocytes from the buffalos ovary and maturing them In Vitro and studying all the hormonal changes.

Collected (38) ovaries are collected from the 20 female buffalo who had slaughtered in the massacre of the Babylon province for the period from 14/05/2014 to 20/12/2014.

The results showed that there was a significant effect (P ≤ 0.05) of age on the total number of oocytes, while the effect of age on the number of good and mature oocytes was not significant ( P≥ 0.05) However , there was a significant effect (P ≤ 0.05) of age in the proportion of mature oocytes.

There was a significant (P≤ 0.05) effect of age on the level of estrogen and progesterone hormones , while no significant difference ( P≥ 0.05) in the level of FSH and LH hormones was found.

The results of the study showed that there was no significant difference( P≥ 0.05) between the absence of the corpus luteum and the total number of oocytes , while there is higher proportion of the good and Mature oocytes with the presence of the corpus luteum, The results of the study showed that the presence of corpus luteum was not significantly (P≥ 0.05) affects the level of studied hormones a( FSH,LH, EstrogenE2 and Progesterone P4) respectively .