تأثير نوع و مكونات الوسط الغذائي و الجزء النباتي في نشوء أصلي الحمضيات فولكامارياناو اليوسفي كليوباترا خارج الجسم الحي

Abstract EN

This study was conducted in tissue culture laboratory of the general company for Horticulture and Foresty/ Ministry of Agriculture/ Iraq from September 2013 to August 2014 to determine the best explants (single nodal segment and tip shoots) and medium (MT,WPM) for in vitro culture initiation the citrus rootstocks (Volkameriana , Cleopatra mandarin ).

Best response was in MT medium that supplemented with 1.0 mg.L-1 BA for Volkameriana rootstock which gave 50% compared with10% for WPM medium for the same rootstock and superiority on Cleopatra mandarin which gave response 30% and 0.0% to same concentrate and two medium respectively.

The results showed high significant difference for the single node at tip shoots in all BA treatments in volkameriana and Cleopatra with increasing concentrate of BA 1.0 and 1.5 mg.L-1 that added to MT medium and WPM supplemented with 0.3 mg/L-1 NAA and 0.5mg/L-1 kin.

that gave the highest response percentage for the single node was %50 for both rootstocks.

While the two concentrate 1.0 and 1.5 mg.L-1 BA which gave a high percentage to the single node response was 60% for two mediums WPM and MT which Was to them 0.5, 40 mg.L-1 from GA3 and ADS respectively.