مستويات الليبتين لدى مريضات متلازمة المبيض متعدد الكيسات و قدرتها على التنبؤ بنتائج عملية الإخصاب في المختبر

Abstract EN

Polycystic ovarian syndrome (PCOS) is a major cause of dysovulatory infertility in women of reproductive age.

Although the pathogenesis of PCOS remains unclear till now, some studies reported that leptin plays a role in the pathogenesis of this syndrome, and that serum and follicular fluid leptin levels may have the ability to predict in vitro fertilization (IVF) outcome in PCOS patients.

This study aimed to determine the levels of leptin in the serum, as well as, in the follicular fluid (FF) of polycystic ovary syndrome (PCOS) patients, and to evaluate the benefit of serum and follicular fluid leptin levels as predictors for IVF outcome in PCOS patients.

The study design is Case- control study.

40 patients with polycystic ovary syndrome and 40 age and weight matched control women were included in this study.

All of them enrolled in the in vitro fertilization (IVF) program.

Blood and FF samples were collected from all subjects on the day of oocyte retrieval.

Leptin levels were measured in serum and FF using enzyme linked immunosorbent assay (ELISA).

Serum leptin levels did not differ between PCOS patients and control group (23.95±11.26 ng/ml, 22.27±11.75 ng/ml, respectively, p=0.514), also Follicular Fluid leptin levels did not differ between PCOS patients and control group (48.39±12.12 ng/ml, 43.27±13.19 ng/ml, respectively, p=0.074).

Serum and FF leptin levels were positively correlated with BMI (P<0.05).

Both serum and FF leptin levels were not significantly correlated to any of the IVF outcomes (P>0.05).

Serum leptin levels did not differ between pregnant and non-pregnant women in both PCOS patients (27.11±9.46 ng/ml, 23.97±13.06 ng/ml, respectively, p=0.474) and control group (20.15±13.81 ng/ml, 21.57±8.08 ng/ml, respectively, p=0.743), and FF leptin levels did not differ between pregnant and non-pregnant women in both PCOS (50.88±9.57 ng/ml, 48.71±12 ng/ml, respectively, p=0.606) and control women (39.62±13.14 ng/ml, 43.99±11.47 ng/ml, respectively, p=0.344).

As conclusion, There is no difference in serum and Follicular fluid leptin levels between PCOS patients and control group, whereas these levels are positively correlated with BMI.

Thus, changes in leptin levels are mainly related to body weight and have no correlation to polycystic ovary syndrome.

Also, serum and FF leptin levels couldn’t be used as predictors for in vitro fertilization (IVF) outcome