تقدير فاعلية إنزيم اللايبيز المنتج من بكتيريا Bacillus subtilis المعزولة من التربة

Abstract EN

Sequence comparisons within the lipase family, in Lipases: Their structure, biochemistry and applications, edited by P Y Woolley & S B Petersen (Cambridge University Press, Cambridge), 1-21 Abstact: The study included the isolation of Bacillus subtilis and diagnosis from Soil specimens of Rizosphere.

A (7) from the Najaf and 7 from Kufa.

The bacterial isolates were amined for capability of producing lipase were grown on Tween-80 agar medium .One isolates are studies, The optimum conditions for lipase producing were defined by measuring the specific activity.

The optimum pH 8for 48 hours at 45 C o, The enzyme was extracted by centrifugation which was filtered by ultrafiltration tube , and was purified by ammonium sulfate precipitation (70% saturation) for lipase .Then were dialysis by dialysis bag and Batch chromatography using DEAE-Cellulose .

The purified lipase had specific activity of 1035.66U/mg with 6.41 purification folds and %18.4 enzymatic yield