دراسة مقارنة لطرق تشخيص أداء الليشمانيات الجلدي

Abstract EN

In Syria, cutaneous leishmaniasis (CL) is diagnosed by conventional techniques using microscopic identification and culture.

Due to their high specificity, they are widely spread.

On the other hand, several drawbacks are still limited their application.

Among these are low sensitivity and the length of the test.

Since the past world researchs showed that kDNA PCR is highly sensitive and specific, the objective of our research was to compare the sensitivity of microscopic examination, culture and kDNA PCR based on local samples.

Materials and Methods: samples were obtained from 70 of out patients with suspected CL referred to Damascus Dermatology hospital.

Each sample was examined through the three methods, microscopic examination, culture and kDNA PCR.

Results: Sensitivity of the three applied methods were: 72.9% for microscopic smear, 68.6% for culture, 100% for the kDNA-PCR method.

Conclusion: The kDNA-PCR method appear to be the most sensitive method for diagnosing CL.

It is therefore especially recommended in the cases where leishmania infection is clinicaly suspected and negative results of microscopy or/and culture are obtaind.