مقارنة مستويات H-FABP و cTnI و CK-MB المصلية في احتشاء العضلة القلبية

Abstract EN

Heart-type fatty acid-binding protein (H-FABP) is a 132 amino acids soluble protein, with a low molecular weight (14 kDa), and it is found abundantly in the myocardiocyte cytosole.

Aim: This study aimed to evaluate the relationship of H-FABP serum concentrations with myocardial infarction (MI), and compare between H-FABP and cardiac Troponin I (cTnI) and Creatine Kinase isoenzyme (CK-MB), which are used in diagnosis.

Materials and Methods: Blood samples were drawn within 24 hours after onset of symptoms in 137 patients admitted to hospital with chest pain.

Eighty seven of them were diagnosed with MI, 30 unstable angina, 4 CAD, 8 ACS, and 8 suffered chest pain for noncardiac reasons.

Also blood samples were drawn of 30 apparently healthy subjects as control group.

Serum samples were used.

Both H-FABP and cTnI were measured using sandwich ELISA assay, whereas CK-MB was measured using kinetic method.

H-FABP was compared in sensitivity and specificity with cTnI and CK-MB.

To eliminate the effect of cutoff point on the comparison between markers, we constructed the receiver operating characteristic curve (ROC) for each marker and compared the area under the curve (AUC) between markers.

Results: H-FABP concentrations in MI group were significantly higher than those in control group.

Within 24 hours after onset Background: Heart-type fatty acid-binding protein (H-FABP) is a 132 amino acids soluble protein, with a low molecular weight (14 kDa), and it is found abundantly in the myocardiocyte cytosole.

Aim: This study aimed to evaluate the relationship of H-FABP serum concentrations with myocardial infarction (MI), and compare between H-FABP and cardiac Troponin I (cTnI) and Creatine Kinase isoenzyme (CK-MB), which are used in diagnosis.

Materials and Methods: Blood samples were drawn within 24 hours after onset of symptoms in 137 patients admitted to hospital with chest pain.

Eighty seven of them were diagnosed with MI, 30 unstable angina, 4 CAD, 8 ACS, and 8 suffered chest pain for noncardiac reasons.

Also blood samples were drawn of 30 apparently healthy subjects as control group.

Serum samples were used.

Both H-FABP and cTnI were measured using sandwich ELISA assay, whereas CK-MB was measured using kinetic method.

H-FABP was compared in sensitivity and specificity with cTnI and CK-MB.

To eliminate the effect of cutoff point on the comparison between markers, we constructed the receiver operating characteristic curve (ROC) for each marker and compared the area under the curve (AUC) between markers.

Results: H-FABP concentrations in MI group were significantly higher than those in control group.

Within 24 hours after onset of symptoms, H-FABP specificity was significantly higher than CK-MB (76%, 64% respectively, P=0.023).

Also H-FABP sensitivity was significantly higher than cTnI in 6 to 12 hours after onset of symptoms (79%, 57% respectively, P=0.046).

The AUC for H-FABP, cTnI, and CK-MB were 0.824, 0.834, 0.780 respectively.

Moreover AUC in the first six hours after onset of symptoms for H-FABP, cTnI, and CK-MB were 0.753, 0.707, 0.493 respectively.

Conclusion: This study has showed that H-FABP is a good marker for myocardial infarction that is competent to cTnI and has better specificity than CK-MB.

Furthermore, H-FABP has better sensitivity than cTnI in 6 to 12 hours after onset of symptoms.