استخلاص و تنقية إنزيم الفيسين من أوراق التين و اختبار كفاءته التحليلية

Abstract EN

The present study aimed to extraction and purification ficin enzyme which extracted from leaves of Ficus and study it's proteolytic efficiency.

Many solutions were used for extraction such as distilled water, sodium chloride (2 and 7%) solution ,Sodium Phosphate with citrate solution 0.1M and pH 7, Ethanol, cold acetone with citrate and phosphate solution and Tris-Hcl 0.1 M, pH 9 in order to find out the best extraction solution.

Tris-HCl 0.1 M pH 9 solution was the best extraction solution which gave the highest specific activity being 93.48unit / mg Protein .

Protein content for the crude enzyme extracts was precipitated by gradual addition of solid ammonium sulphate to final saturation of 20 -90% the activity being 688.95 unit / mg Purification Folds 6 time and 26.12% as a yield .

Gel filtration followed by Sephadex G-100 and ion exchange chromatography through DEAE Sephadex A-50 columin were used to complete purification of ficin and tested proteolytic activity in each step of purification which exhibit 2733.36, Purification Folds 23.90 time and 15.43% as a yield.

The purity of ficin was examined by poly acryl amide gel electrophoresis.