Generation and characterization of Second antibodies (rabbit anti-mouse) for use as a separating agent
Joint Authors
Shafiq, H. M.
Bayyumi, A. S.
Mihani, N. L.
Ubayd, Narin Hafiz
Source
Arab Journal of Nuclear Sciences and Applications
Issue
Vol. 49, Issue 3 (31 Jul. 2016), pp.127-136, 10 p.
Publisher
The Egyptian Society of Nuclear Science and Applications
Publication Date
2016-07-31
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Zoology
Materials Science , Minerals
Abstract EN
The objective of this study is the production of second antibody (rabbit anti-mouse IgG) 2nd Ab by immunization of rabbits with immunoglobulin IgG purified from mouse.
The present study involves the production of anti-AFP polyclonal in Balb/C mice which are used as a 1st Ab in liquid phase RIA.
AFP standards were prepared.
The tracers (125I-AFP and 125I-AFP-MoAb) were prepared by iodogen method.
The produced 2nd Ab provides a great efficiency to be used as a separating agent at dilution 1/20.
American Psychological Association (APA)
Ubayd, Narin Hafiz& Shafiq, H. M.& Bayyumi, A. S.& Mihani, N. L.. 2016. Generation and characterization of Second antibodies (rabbit anti-mouse) for use as a separating agent. Arab Journal of Nuclear Sciences and Applications،Vol. 49, no. 3, pp.127-136.
https://search.emarefa.net/detail/BIM-724288
Modern Language Association (MLA)
Ubayd, Narin Hafiz…[et al.]. Generation and characterization of Second antibodies (rabbit anti-mouse) for use as a separating agent. Arab Journal of Nuclear Sciences and Applications Vol. 49, no. 3 (Jul. 2016), pp.127-136.
https://search.emarefa.net/detail/BIM-724288
American Medical Association (AMA)
Ubayd, Narin Hafiz& Shafiq, H. M.& Bayyumi, A. S.& Mihani, N. L.. Generation and characterization of Second antibodies (rabbit anti-mouse) for use as a separating agent. Arab Journal of Nuclear Sciences and Applications. 2016. Vol. 49, no. 3, pp.127-136.
https://search.emarefa.net/detail/BIM-724288
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 135-136
Record ID
BIM-724288
