تطوير طريقة تحليلية كروماتوغرافية سائلة عالية الأداء لتعيين هيوسين بوتيل برومايد في الشرابات السائلة

Abstract EN

A simple and rapid analytical method for routine quantification of Hyoscine butyl bromide (scopolamine butyl bromide) in liquid oral formulations by highperformance liquid chromatography was developed and validated.

The oral solution samples can be directly analyzed by reversed-phase HPLC on Intersil ODS-3 column (4.6 mm x 250 mm, i.d., 5-micron particle size) with acetonitrilephosphate buffer of pH= 6.80± 0.05 (30:70 v/v) respectively as a mobile phase that has a flow rate of 1.5 mL /min.

UV Detection was carried out at 210 nm.

The method was linear over the selected concentration and ranged from 0.02 to 0.14 mg/mL (r2 = 0.9992) and the detection limit was 0.0012 mg/mL.

The mean percent recoveries ranged from 99.0% to 100.09%.

The results demonstrated good linearity, accuracy, and precision.

The method was applied to determine commercial liquid oral formulations.