الأهمية الطبية الشرعية لبعض الواسمات الحيوية الكيميائية (الإسترات الإيتيلية للحموض الدسمة)‎ لتحديد الكحولية : دراسة مقارنة في الحركية السمية للإيتانول و الاستفادة من نفوذه إلى كرة العين (الخلط الزجاجي)‎ و تطبيقاته في مجال الطب الشرعي

Abstract EN

Background & Objective: The concentration of ethanol in blood and urine provides important evidence in criminal and civil litigation when alcohol- related crimes are investigated (e.g., drunk driving )Therefore.

Ethanol analysis is the most frequently performed assay in forensic toxicology laboratories .

.However, the risk for artifactual formation of ethanol, especially in postmortem specimens, always needs to be considered .

It is often difficult to distinguish between postmortem ethanol production and antemortem alcohol ingestion .

Ethanol synthesis could be produced by many species of bacteria or yeasts ,which is also influenced as storage temperature, and the interval between death and autopsy increase .

Purpose of the study:To focus on a reliable method of analysis depending on the pharmacokinetcs of ethanol,and to choose the appropriate biological fluid for alcohol analysis .

Methods & Materials: The use of vitreous humour and the modifide microdifusion cell proved to be useful permitting the limitation of the interference in postmortem ethanol evaluation.

Results: An interesting modification in the microdifusion cell used for the detection of volatiles proved to be useful permitting measurements in emergency situations and decreasing the probability of postmortem ethanol synthesis produced by many species of bacteria or yeasts ,which is also influenced as storage temperature, and the interval between death and autopsy increase .

Conclusion: analysis of vitreous humour is useful to corroborate a postmortem blood alcohol and assist in distinguishing antemortem intoxication from post mortem alcohol production.

In most cases the specimen is easily obtained and can be sampled without a full autopsy .

Vitreous humour is a clear ,serous fluid which is easy to work anatically .Its anatomically isolated protects it from bacterial putrefaction