تقصي جين هيستون H2B و H3 في الليشمانية المدارية
Other Title(s)
Investigation of H2B and H3 genes in leishmania tropica
Joint Authors
Source
Issue
Vol. 8, Issue 1-2 (31 Jul. 2016), pp.55-63, 9 p.
Publisher
Syrian Clinical Laboratory Association
Publication Date
2016-07-31
Country of Publication
Syria
No. of Pages
9
Main Subjects
Topics
- Parasitology
- Infectious diseases
- Skin diseases
- Parasitic diseases
- Leishmaniasis
- Gene therapy
- Syria
- Genes
- Infection
Abstract EN
Cutaneous Leishmaniasis caused by Leishmania tropica is one of the most important health issues in Syria, which exacerbated after what is happening in Syria.
Prevention from Leishmaniasis is very important to prevent the high prevalence of infection in Syria.
DNA vaccine is a promising hope in this field.
Nucleosomal core histones are very important for the parasites life, so we choose two of their genes, H2B and H3 genes, to design a DNA vaccine against the infection with L.
tropica.
Our study aimed to investigate the existence of H2B and H3 genes in a Syrian strain of L.
tropica promastigotes and their expression.
We isolated DNA and RNA from these parasites, synthesized cDNA, design the necessary primers for gene`s amplification, and standardization of PCR condtions.
We proved existence of these genes in L.
tropica promastigotes.
Their sizes of H2B and H3 genes were 336 and 393 bp, respectively.
Expression was also proved in these parasites, through proving their existence in the extracted RNA of Leishmania tropica promastigotes and amplification the complementary DNA of these genes.
American Psychological Association (APA)
الحبش، مروة ومعروف، محمد. 2016. تقصي جين هيستون H2B و H3 في الليشمانية المدارية. مجلة التشخيص المخبري،مج. 8، ع. 1-2، ص ص. 55-63.
https://search.emarefa.net/detail/BIM-746617
Modern Language Association (MLA)
الحبش، مروة ومعروف، محمد. تقصي جين هيستون H2B و H3 في الليشمانية المدارية. مجلة التشخيص المخبري مج. 8، ع. 1-2 (تموز 2016)، ص ص. 55-63.
https://search.emarefa.net/detail/BIM-746617
American Medical Association (AMA)
الحبش، مروة ومعروف، محمد. تقصي جين هيستون H2B و H3 في الليشمانية المدارية. مجلة التشخيص المخبري. 2016. مج. 8، ع. 1-2، ص ص. 55-63.
https://search.emarefa.net/detail/BIM-746617
Data Type
Journal Articles
Language
Arabic
Notes
يتضمن مراجع ببليوجرافية : ص. 62-63
Record ID
BIM-746617